Document Detail

Direct pulp capping with mineral trioxide aggregate: an immunohistologic comparison with calcium hydroxide in rodents.
MedLine Citation:
PMID:  20416425     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: The aim was to evaluate the proliferation of pulp cells 1, 3, and 7 days after direct pulp capping with ProRoot MTA (MTA) and to compare the results with calcium hydroxide (Ca(OH)(2)). METHODS: An occlusal cavity was prepared in 36 molar teeth of 18 Wistar rats. Then MTA or Ca(OH)(2) was placed on the exposed pulp. All cavities were restored with composite. After 1, 3, and 7 days the animals were killed. One hour before scarification 5-bromo-2'-deoxyuridine (BrdU) was injected into the intraperitoneal cavity for immunohistologic analysis. BrdU was incorporated into the cell nucleus during the S phase of the cell cycle. Proliferating cells were tagged and counted by using alkaline phosphatase and anti-alkaline phosphatase antibody staining. Three animals (6 molar teeth) served as controls and were not further treated. The number of the tagged cells was statistically analyzed by comparing the results of the 3 groups. A Bonferroni correction was performed, because the data of the Ca(OH)(2)- group was used 3 times for pairwise comparison. RESULTS: The marked cells were identified as fibroblasts, endothelial cells (after 1, 3, and 7 days), and Höhl cells (after 7 days). The MTA group showed a similar amount of Höhl cells when compared with the Ca(OH)(2) group (P > .05). One day and 7 days after capping, no significant differences were observed between the 2 tested groups and the controls (P > .05). After 3 days, significantly more cells were stained in the MTA and Ca(OH)(2) groups than in the control group (P < .016). CONCLUSIONS: Immunohistologic analysis demonstrated that MTA showed similar results when compared with Ca(OH)(2) within the first week after direct pulp capping.
Till Dammaschke; Udo Stratmann; Philipp Wolff; Darius Sagheri; Edgar Schäfer
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Publication Detail:
Type:  Journal Article     Date:  2010-03-15
Journal Detail:
Title:  Journal of endodontics     Volume:  36     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  814-9     Citation Subset:  D    
Copyright Information:
Copyright (c) 2010 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Department of Operative Dentistry, Westphalian Wilhelms-University, Münster, Germany.
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MeSH Terms
Aluminum Compounds / therapeutic use*
Bromodeoxyuridine / metabolism
Calcium Compounds / therapeutic use*
Calcium Hydroxide / therapeutic use*
Dental Pulp Capping / methods*
Drug Combinations
Endothelial Cells / metabolism
Fibroblasts / metabolism
Mitotic Index
Oxides / therapeutic use*
Rats, Wistar
Root Canal Filling Materials / therapeutic use*
Silicates / therapeutic use*
Reg. No./Substance:
0/Aluminum Compounds; 0/Calcium Compounds; 0/Drug Combinations; 0/Oxides; 0/Root Canal Filling Materials; 0/Silicates; 0/mineral trioxide aggregate; 1305-62-0/Calcium Hydroxide; 59-14-3/Bromodeoxyuridine

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