| Direct phenotyping of human apolipoprotein E in plasma: application to population frequency distribution in Paris (France). | |
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MedLine Citation:
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PMID: 8330879 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apolipoprotein E (Apo E) is a component of VLDL and HDL and plays a significant role in the regulation of cholesterol concentration. An improvement in isoelectric focusing for Apo E phenotyping is presented: the plasma Apo E was dissociated from lipoproteins by the use of Tween 20; the optimal concentration of type V neuraminidase was determined (1 U/ml); up to 48 samples were analyzed per plate and revealed by immunoblotting. Using this method, we have determined Apo E phenotypes and estimated their association with total cholesterol and Apo B levels in 498 healthy blood donors in Paris (France). The relative frequencies of Apo E alleles epsilon 2, epsilon 3 and epsilon 4 in this population were 0.079, 0.801 and 0.120, respectively. The association between Apo E phenotypes and concentration of Apo B-containing lipoproteins was confirmed (Apo B (g/l): E4/E3 subjects, 1.10 +/- 0.29; E3/E2 subjects, 0.93 +/- 0.22; both significantly different from E3/E3 subjects, 0.99 +/- 0.28). Total cholesterol (mmol/l): E4/E3 subjects, 5.43 +/- 1.15; E3/E2 subjects, 4.79 +/- 0.83; both significantly different from E3/E3 subjects, 5.03 +/- 1.11. |
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Authors:
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S Bailleul; R Couderc; V Landais; G Lefèvre; D Raichvarg; J Etienne |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Human heredity Volume: 43 ISSN: 0001-5652 ISO Abbreviation: Hum. Hered. Publication Date: 1993 May-Jun |
Date Detail:
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Created Date: 1993-08-18 Completed Date: 1993-08-18 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0200525 Medline TA: Hum Hered Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 159-65 Citation Subset: IM |
Affiliation:
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Service de Biochimie, Hôpital Tenon, Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Alleles Apolipoproteins B / blood Apolipoproteins E / blood, genetics* Blood Donors Cholesterol / blood Female Gene Frequency* Humans Immunoblotting Immunophenotyping Isoelectric Focusing Male Middle Aged Neuraminidase Paris Phenotype Polymorphism, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins B; 0/Apolipoproteins E; 57-88-5/Cholesterol; EC 3.2.1.18/Neuraminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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