Document Detail


Direct and phagocyte-mediated lipid peroxidation of lung surfactant by group B streptococci.
MedLine Citation:
PMID:  11147315     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In newborn infants, group B streptococci (GBS) often cause pneumonia, with polymorphonuclear leukocytes (PMN) migrating into the lungs. Because surfactant therapy may be needed in such patients, we evaluated the interaction between GBS or GBS-stimulated PMN and a surfactant preparation (Curosurf) in vitro. The superoxide production of GBS strains or GBS-activated PMN was measured, using the nitroblue tetrazolium (NBT) test and the subsequent lipid peroxidation (LPO) as the content of malondialdehyde (MDA) and 4-hydroxyalkenals (4-HNE). The growth of GBS in surfactant was determined and related to the LPO. Finally, the effect of LPO on surfactant activity, caused by GBS-stimulated PMN, was assessed by measuring dynamic surface tension in a pulsating bubble surfactometer. Curosurf diminished the NBT reduction by both live GBS and GBS-stimulated PMN. Surfactant was peroxidized by reactive oxygen species (ROS) from both GBS and GBS-stimulated PMN in a time-dependent manner. Vitamin E significantly reduced the peroxidation level of surfactant in both cases. Surfactant peroxidation was associated with a reduction in the number of live bacteria. The biophysical activity of Curosurf was impaired by GBS-stimulated PMN, as reflected by increased minimum surface tension during cyclic compression. These findings indicate that Curosurf undergoes LPO by ROS produced by GBS and/or PMN. We speculate that exogenous surfactant preparations should be supplemented with vitamin E or another antioxidant, when given to infants with GBS pneumonia.
Authors:
R K Bouhafs; P Rauprich; E Herting; A Schröder; B Robertson; C Jarstrand
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lung     Volume:  178     ISSN:  0341-2040     ISO Abbreviation:  Lung     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-05     Completed Date:  2001-02-22     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  7701875     Medline TA:  Lung     Country:  United States    
Other Details:
Languages:  eng     Pagination:  317-29     Citation Subset:  IM    
Affiliation:
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Division of Oral and Clinical Bacteriology, Karolinska Institutet, Huddinge Hospital, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Agents*
Humans
Lipid Peroxidation*
Malondialdehyde / metabolism
Neutrophil Activation / physiology*
Neutrophils / metabolism*,  microbiology
Phospholipids*
Pulmonary Surfactants / metabolism*
Reactive Oxygen Species / metabolism*
Streptococcus agalactiae / physiology*
Superoxides / metabolism
Time Factors
Vitamin E / pharmacology
Chemical
Reg. No./Substance:
0/Biological Agents; 0/Phospholipids; 0/Pulmonary Surfactants; 0/Reactive Oxygen Species; 11062-77-4/Superoxides; 1406-18-4/Vitamin E; 542-78-9/Malondialdehyde; KE3U2023NP/poractant alfa

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