| Direct observations of synapses between GABA-immunoreactive boutons and identified spinocervical tract neurons in the cat's spinal cord. | |
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MedLine Citation:
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PMID: 1856328 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three spinocervical tract neurons in adult cats were physiologically characterized and intracellularly labelled with horseradish peroxidase. The neurons were reconstructed and examined with the light microscope and were prepared for postembedding immunochemical analysis by using an antiserum which specifically recognizes GABA in glutaraldehyde-fixed tissue. Semithin sections were tested and examined with the light microscope. Somata, proximal, and distal dendrites of all three cells were associated with numerous punctate GABA-immunoreactive structures. Immunoreactive perikarya of small neurons in the vicinity of spinocervical tract cells were also observed. Ultrastructural analysis, with the immunogold technique, revealed that somata and proximal dendrites of all three neurons received synaptic contacts (about 37% of total synapses) from GABA-immunoreactive boutons and that distal dendrites were also associated with substantial numbers of immunoreactive structures (about 27% of synapses). Immunoreactive boutons were small (about 1 micron in diameter), contained irregularly shaped agranular vesicles, and formed symmetrical synaptic junctions with identified neurons. An additional group of immunoreactive boutons was observed to be associated with one of the cells only; these contained many large dense-core vesicles in addition to small agranular vesicles. Boutons containing round agranular vesicles and flattened agranular vesicles were not observed to be immunoreactive. The evidence supports the idea that much of the postsynaptic inhibition observed in spinocervical tract neurons is mediated by GABA and that even the most distal dendrites of these neurons receive inhibitory inputs. |
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Authors:
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D J Maxwell; W M Christie; A D Short; A G Brown |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of comparative neurology Volume: 307 ISSN: 0021-9967 ISO Abbreviation: J. Comp. Neurol. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-08-28 Completed Date: 1991-08-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0406041 Medline TA: J Comp Neurol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 375-92 Citation Subset: IM |
Affiliation:
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Department of Preclinical Veterinary Sciences, University of Edinburgh, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cats Dendrites / ultrastructure Electrophysiology Horseradish Peroxidase / diagnostic use Image Processing, Computer-Assisted Immunohistochemistry Microscopy, Electron Neurons / physiology, ultrastructure* Spinal Cord / cytology*, physiology, ultrastructure Synapses / physiology, ultrastructure* gamma-Aminobutyric Acid / immunology, physiology* |
| Chemical | |
Reg. No./Substance:
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56-12-2/gamma-Aminobutyric Acid; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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