| Direct observation of radial intracellular PO2 gradients in a single cardiomyocyte of the rat. | |
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MedLine Citation:
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PMID: 9688918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the present study was to directly visualize radial gradients of intracellular PO2 in a single individual cardiomyocyte isolated from the rat ventricle. Microspectrophotometry with the use of cytosolic myoglobin as an oxygen probe was conducted at 410 nm. When the quiescent cell was incubated with 1 microM carbonyl cyanide m-chlorophenylhydrazone to increase oxygen consumption approximately eightfold, gradual decreases in myoglobin oxygen saturation (SMb) were demonstrated toward the core of the cell, whereas these decreases disappeared when the cell was treated with 2 mM NaCN. These results highlighted the importance of diffusional oxygen transport in determining intracellular oxygenation in cardiac cells. From the measured SMb, we assessed the profile of radial changes in intracellular PO2 at the mean SMb comparable to that in vivo ( approximately 0.5). Quite steep PO2 gradients were demonstrated in the vicinity of the sarcolemma that were rapidly attenuated toward the cell core. These radial profiles of intracellular PO2 demonstrate the significance of myoglobin-facilitated diffusion of oxygen. Furthermore, the shallow gradients of PO2 near the center of the cell might arise from partial depression of oxygen consumption near the cell core. |
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Authors:
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E Takahashi; K Sato; H Endoh; Z L Xu; K Doi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of physiology Volume: 275 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1998 Jul |
Date Detail:
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Created Date: 1998-08-27 Completed Date: 1998-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H225-33 Citation Subset: IM |
Affiliation:
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Department of Physiology, Yamagata University School of Medicine, Yamagata 990-9585, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology Cells, Cultured Cytosol / metabolism Diffusion Myocardium / cytology, metabolism* Myoglobin / metabolism Oxygen / analysis, metabolism* Oxygen Consumption / drug effects Partial Pressure Rats Rats, Sprague-Dawley Sarcolemma / metabolism Spectrophotometry / methods |
| Chemical | |
Reg. No./Substance:
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0/Myoglobin; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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