Document Detail


Direct measurement of nipradilol-derived nitric oxide in the vascular wall of canine femoral arteries.
MedLine Citation:
PMID:  16025369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nipradilol (NP: 3,4-dihydro-8-[2-hydroxy-3-isopropylamino]propoxy-3-nitroxy-2H-1-benzopyran) shows not only beta-adrenoreceptor-blocking effects but also nitroglycerin-like vasodilatory action. We aimed to directly measure NP-derived nitric oxide (NO) in the vascular wall. An NO-sensitive microelectrode was inserted into the vascular media (the vasodilatory action site of NO) of isolated perfused canine femoral arteries. Each vessel was perfused with 15 microM NP in the presence or absence of 1 mM N-ethylmaleimide (NEM; a thiol alkylator). Intravascular-wall NO concentration increased 181+/-34 nM during NP perfusion (P<0.001 vs basal, n=10) with an average base-to-peak reaction time of 1.5+/-0.1 min (P<0.0001, n=8). Concomitant perfusion of NEM with NP attenuated the intravascular-wall NO production significantly (P<0.0001 vs NP only). It is concluded that NP is metabolized to NO in the vascular wall of an isolated canine femoral artery in large part through a metabolic process involving thiols with a base-to-peak reaction time of about 1.5 min.
Authors:
Seiichi Mochizuki; Yoko Chiba; Osamu Hiramatsu; Hiroyuki Tachibana; Hiroshi Nakamoto; Eiji Toyota; Yasuo Ogasawara; Fumihiko Kajiya
Related Documents :
3968639 - Effects of nisoldipine on cardiocirculatory dynamics and cardiac output distribution in...
2390979 - Dependence on exercise intensity of changes in electrolyte secretion from the skin samp...
1423969 - Serotonin selectively aggravates subendocardial ischemia distal to a coronary artery st...
3596339 - Effects of exercise on mesenteric blood flow in man.
1576839 - Regional flow responses to exercise.
15962189 - Subacute effects of a maximal exercise bout on endothelium-mediated vasodilation in hea...
7399989 - Energy expenditure during oxygen deficit of submaximal concentric and eccentric exercise.
17909409 - Oxygen-uptake efficiency slope as a determinant of fitness in overweight adolescents.
12957629 - 'in vitro' antioxidant and photoprotective properties and interaction with model membra...
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Heart and vessels     Volume:  20     ISSN:  0910-8327     ISO Abbreviation:  Heart Vessels     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-07-18     Completed Date:  2006-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511258     Medline TA:  Heart Vessels     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  175-8     Citation Subset:  IM    
Affiliation:
Department of Medical Engineering, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama, 701-0192, Japan. mochi@me.kawasaki-m.ac.jp
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adrenergic beta-Antagonists / pharmacology*
Animals
Dogs
Femoral Artery / metabolism*
Microelectrodes
Nitric Oxide / metabolism*
Propanolamines / pharmacology*
Vasodilator Agents / pharmacology*
Chemical
Reg. No./Substance:
0/Adrenergic beta-Antagonists; 0/Propanolamines; 0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 81486-22-8/K 351

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rapid progression of atherosclerotic coronary artery disease in patients with human immunodeficiency...
Next Document:  Positron emission tomography/computed tomography improves diagnostics of inflammatory arteritis.