Document Detail


Direct measurement of intracellular pressure.
MedLine Citation:
PMID:  24894836     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A method to directly measure the intracellular pressure of adherent, migrating cells is described in this unit. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode, as well as the application of the method to cells migrating inside three-dimensional (3-D) extracellular matrix (ECM). Curr. Protoc. Cell Biol. 63:12.9.1-12.9.9. © 2014 by John Wiley & Sons, Inc.
Authors:
Ryan J Petrie; Hyun Koo
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Publication Detail:
Type:  Journal Article     Date:  2014-06-03
Journal Detail:
Title:  Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]     Volume:  63     ISSN:  1934-2616     ISO Abbreviation:  Curr Protoc Cell Biol     Publication Date:  2014  
Date Detail:
Created Date:  2014-06-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101287856     Medline TA:  Curr Protoc Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12.9.1-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 John Wiley & Sons, Inc.
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