Document Detail


Direct measurement of creatine kinase-MB activity in serum after extraction with a monoclonal antibody specific to the MB isoenzyme.
MedLine Citation:
PMID:  3955814     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fusion of splenocytes from A/J mice immunized by creatine kinase (EC 2.7.3.2)-MB isoenzyme (CK-MB) with SP2/0-Ag14 myeloma cell line generated hybridomas producing monoclonal antibodies specific to CK-MB. One of these monoclonal antibodies ("Conan-MB") was used to develop a direct assay for CK-MB activity. In the assay, serum is incubated for 30 min at room temperature with "Conan-MB" immobilized on latex beads. The beads are then washed, and CK-MB activity bound to the antibody is measured after incubation with CK enzyme reagent for 30 min at 37 degrees C. Results with the assay correlated well (r = 0.997) with those for CK-MB concentration as measured by a two-site immunoassay. Neither CK-MM, CK-BB, mitochondrial CK, nor a hemolysate of erythrocytes interfered. Use of this unique monoclonal antibody allows rapid, precise, and direct determination of CK-MB activity.
Authors:
H C Vaidya; Y Maynard; D N Dietzler; J H Ladenson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  32     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-05-20     Completed Date:  1986-05-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  657-63     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / isolation & purification
Antibody Specificity
Ascites / immunology
Creatine Kinase / blood*,  immunology
Electrophoresis, Agar Gel
Female
Humans
Immunoassay / methods
Isoenzymes
Latex
Mice
Mice, Inbred A
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Isoenzymes; 0/Latex; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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