| Direct measurement of creatine kinase-MB activity in serum after extraction with a monoclonal antibody specific to the MB isoenzyme. | |
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MedLine Citation:
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PMID: 3955814 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fusion of splenocytes from A/J mice immunized by creatine kinase (EC 2.7.3.2)-MB isoenzyme (CK-MB) with SP2/0-Ag14 myeloma cell line generated hybridomas producing monoclonal antibodies specific to CK-MB. One of these monoclonal antibodies ("Conan-MB") was used to develop a direct assay for CK-MB activity. In the assay, serum is incubated for 30 min at room temperature with "Conan-MB" immobilized on latex beads. The beads are then washed, and CK-MB activity bound to the antibody is measured after incubation with CK enzyme reagent for 30 min at 37 degrees C. Results with the assay correlated well (r = 0.997) with those for CK-MB concentration as measured by a two-site immunoassay. Neither CK-MM, CK-BB, mitochondrial CK, nor a hemolysate of erythrocytes interfered. Use of this unique monoclonal antibody allows rapid, precise, and direct determination of CK-MB activity. |
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Authors:
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H C Vaidya; Y Maynard; D N Dietzler; J H Ladenson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical chemistry Volume: 32 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1986 Apr |
Date Detail:
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Created Date: 1986-05-20 Completed Date: 1986-05-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 657-63 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / isolation & purification Antibody Specificity Ascites / immunology Creatine Kinase / blood*, immunology Electrophoresis, Agar Gel Female Humans Immunoassay / methods Isoenzymes Latex Mice Mice, Inbred A |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Isoenzymes; 0/Latex; EC 2.7.3.2/Creatine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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