| Direct manipulation of intracellular stress fibres using a hook-shaped AFM probe. | |
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MedLine Citation:
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PMID: 20798461 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atomic force microscopy (AFM) is a highly successful technique for imaging nanometre-sized samples and measuring pico- to nano-newton forces acting between atoms and molecules. When it comes to the manipulation of larger samples with forces of tens and hundreds of nano-newtons, however, the present chemistry-based modification protocols for functionalizing AFM cantilevers to achieve the formation of covalent/non-covalent linkages between the AFM probe and the sample surface do not produce strong enough bonds. For the purpose of measuring the fracture strength and other mechanical properties of stress fibres (SFs) in living as well as semi-intact fibroblast cells, we fabricated an AFM probe with a hooking function by focused ion beam technology and used the AFM probe hook to capture, pull and eventually sever a chosen SF labelled with green or red fluorescent protein. |
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Authors:
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Shinichi Machida; Takahiro Watanabe-Nakayama; Ichiro Harada; Rehana Afrin; Tomonobu Nakayama; Atsushi Ikai |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-27 |
Journal Detail:
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Title: Nanotechnology Volume: 21 ISSN: 1361-6528 ISO Abbreviation: Nanotechnology Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-27 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101241272 Medline TA: Nanotechnology Country: England |
Other Details:
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Languages: eng Pagination: 385102 Citation Subset: IM |
Affiliation:
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Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa, Japan. smachida@bio.titech.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Fibroblasts / ultrastructure* Green Fluorescent Proteins / analysis Luminescent Proteins / analysis Mechanical Phenomena Microscopy, Atomic Force / instrumentation*, methods Rats Stress Fibers / chemistry, ultrastructure* |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 0/red fluorescent protein; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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