Document Detail


Direct isolation of genes encoded within a homogeneously staining region by chromosome microdissection.
MedLine Citation:
PMID:  8090779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification of genes involved in recurring chromosome rearrangements has provided significant insight into the molecular basis of malignancy. We describe here a strategy combining chromosome microdissection and hybrid selection for the direct isolation of chromosome region-specific genes. We modeled this strategy by using sequences recovered from the microdissection of a homogeneously staining region to allow isolation of genes that were overexpressed and present at high copy number within the homogeneously staining region, including the direct isolation of two genes encoded within a 12q homogeneously staining region found in the osteosarcoma cell line OsA-CL. Although first applied to amplified genes, this strategy should be applicable to the isolation of cDNAs from any chromosomal region.
Authors:
Y A Su; J M Trent; X Y Guan; P S Meltzer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  91     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1994 Sep 
Date Detail:
Created Date:  1994-10-14     Completed Date:  1994-10-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9121-5     Citation Subset:  IM    
Affiliation:
Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, MD 20892.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U09408;  U09409
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes / ultrastructure*
Chromosomes, Human, Pair 12*
Cloning, Molecular / methods*
DNA Primers / chemistry
DNA, Complementary / genetics
DNA, Neoplasm / genetics
Gene Amplification*
Gene Expression
Gene Library
Genes
Genomic Library*
Humans
Molecular Sequence Data
Osteosarcoma / genetics*
RNA, Messenger / genetics
Grant Support
ID/Acronym/Agency:
CA-29476/CA/NCI NIH HHS; CA-56725/CA/NCI NIH HHS; P30-HG-00209/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/DNA, Neoplasm; 0/RNA, Messenger
Comments/Corrections

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