Document Detail


Direct interaction of tumor suppressor CEACAM1 with beta catenin: identification of key residues in the long cytoplasmic domain.
MedLine Citation:
PMID:  18445773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CEACAM1-4L (carcinoembryonic antigen cell adhesion molecule 1, with 4 extracellular Ig-like domains and a long, 71 amino acid cytoplasmic domain) is expressed in epithelial cells and activated T-cells, but is down-regulated in most epithelial cell cancers and T-cell leukemias. A highly conserved sequence within the cytoplasmic domain has ca 50% sequence homology with Tcf-3 and -4, transcription factors that bind beta-catenin, and to a lesser extent (32% homology), with E-cadherin that also binds beta-catenin. We show by quantitative yeast two-hybrid, BIAcore, GST-pull down, and confocal analyses that this domain directly interacts with beta-catenin, and that H-469 and K-470 are key residues that interact with the armadillo repeats of beta-catenin. Jurkat cells transfected with CEACAM1-4L have 2-fold less activity in the TOPFLASH reporter assay, and in MCF7 breast cancer cells that fail to express CEACAM1, transfection with CEACAM1 and growth in Ca2+ media causes redistribution of beta-catenin from the cytoplasm to the cell membrane, demonstrating a functional role for the long cytoplasmic domain of CEACAM1 in regulation of beta-catenin activity.
Authors:
Lan Jin; Yun Li; Charng-Jui Chen; Mark A Sherman; Keith Le; John E Shively
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-04-29
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  233     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-24     Completed Date:  2008-07-24     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  849-59     Citation Subset:  IM    
Affiliation:
Division of Immunology, Beckman Research Institute of the City of Hope, 1450 E. Duarte Rd., Duarte, CA 91010, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Antigens, CD / analysis,  metabolism*
Breast Neoplasms / metabolism,  pathology
Cell Adhesion Molecules / analysis,  metabolism*
Humans
Jurkat Cells
Leukemia, T-Cell / metabolism,  pathology
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Interaction Domains and Motifs*
Signal Transduction
T-Lymphocytes / metabolism*,  pathology
Transcriptional Activation
Transfection
Tumor Cells, Cultured
beta Catenin / analysis,  metabolism*
Grant Support
ID/Acronym/Agency:
CA 84202/CA/NCI NIH HHS; R01 CA084202-08/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/CD66 antigens; 0/Cell Adhesion Molecules; 0/beta Catenin
Comments/Corrections

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