Document Detail


Direct and indirect immunolabelling of HeLa cells with quantum dots.
MedLine Citation:
PMID:  18452136     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With excellent optical properties, quantum dots (QDs) have been made as attractive molecular probes for labelling cells in biological research. In this study high-quality CdSe QDs prepared in a paraffin-oleic acid system were used as fluorescent labels in direct and indirect detection of carcinoembryonic antigen (CEA), a cancer marker expressed on the surface of HeLa cells. The primary antibody (Ab) (rabbit anti-CEA8) and secondary Ab (goat anti-rabbit IgG) were covalently linked to carboxyl-functioned CdSe QDs, and both the QDs-antibody and QDs-IgG probes were successfully used to label HeLa cells. The present study demonstrates the practicability of CdSe QDs as an attractive type of fluorescent labels for biological applications such as protein probes and cell imaging.
Authors:
Dongzhi Yang; Qifan Chen; Wenxing Wang; Shukun Xu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  23     ISSN:  1522-7243     ISO Abbreviation:  Luminescence     Publication Date:    2008 May-Jun
Date Detail:
Created Date:  2008-05-22     Completed Date:  2008-07-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  England    
Other Details:
Languages:  eng     Pagination:  169-74     Citation Subset:  IM    
Copyright Information:
(c) 2008 John Wiley & Sons, Ltd.
Affiliation:
Department of Chemistry, Northeastern University, Shenyang 110004, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / diagnostic use*
Cadmium Compounds
Carcinoembryonic Antigen / analysis
Fluorescent Dyes
Hela Cells
Humans
Immunoassay / methods*
Molecular Probe Techniques*
Quantum Dots*
Selenium Compounds
Chemical
Reg. No./Substance:
0/Antibodies; 0/Cadmium Compounds; 0/Carcinoembryonic Antigen; 0/Fluorescent Dyes; 0/Selenium Compounds; 1306-24-7/cadmium selenide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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