Document Detail

Direct immunofluorescence of skin biopsy: perspective of an immunopathologist.
MedLine Citation:
PMID:  20228544     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: By direct immunofluorescence (DIF), presence of immune complexes in the skin biopsy at various locations such as the dermo-epidermal junction, dermal blood vessels, etc. help to arrive at a diagnosis. AIMS: (1) To study the role of DIF in confirmation or exclusion of diseases involving skin vis-à-vis histopathology and clinical diagnosis, (2) to describe the annual spectrum of dermatologic conditions that present to a tertiary referral center and require DIF examination of skin biopsy for confirmation of diagnosis. METHODS: A total of 267 biopsies received over a period of 16 months in the Department of Immunopathology were analyzed along with clinical and histopathological details and the correlation between them was studied. RESULTS: DIF was positive in 204 skin biopsies. Of these, 127 biopsies showed good clinico-immuno-histopathological correlation. In 10 cases, only DIF could clinch the diagnosis. In another nine cases, immune deposits were noted, which were unexpected in light of clinical and histopathological diagnosis. The most common skin involvement was seen in vasculitides. DIF was, however, non-contributory in lesions like erythema multiformè, post Kala-azar dermal leishmaniasis, sarcoidosis, lupus vulgaris, pyoderma gangrenosum and prurigo nodularis. CONCLUSION: The DIF of skin in conjunction with histopathology gives the best diagnostic yield. It is invaluable in confirming the diagnosis of small vessel vasculitides and bullous lesions of skin and can be used as an additional tool to pinpoint the diagnosis of systemic and localized autoimmune diseases involving the skin.
Ranjana Walker Minz; Seema Chhabra; S Singh; B D Radotra; Bhushan Kumar
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Indian journal of dermatology, venereology and leprology     Volume:  76     ISSN:  0973-3922     ISO Abbreviation:  Indian J Dermatol Venereol Leprol     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7701852     Medline TA:  Indian J Dermatol Venereol Leprol     Country:  India    
Other Details:
Languages:  eng     Pagination:  150-7     Citation Subset:  IM    
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
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MeSH Terms
Child, Preschool
Fluorescent Antibody Technique, Direct / methods
Immunologic Techniques
Middle Aged
Skin / immunology*,  pathology*
Skin Diseases / diagnosis,  immunology*,  pathology*
Young Adult

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