| Direct identification of Staphylococcus aureus from positive blood culture bottles. | |
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MedLine Citation:
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PMID: 12574309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targeting Staphylococcus aureus 16S rRNA is a novel method for direct identification of S. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison of S. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity of S. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems. |
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Authors:
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Kenneth Oliveira; Stephen M Brecher; Annette Durbin; Daniel S Shapiro; Donald R Schwartz; Paola C De Girolami; Joanna Dakos; Gary W Procop; Deborah Wilson; Chad S Hanna; Gerhard Haase; Heidrun Peltroche-Llacsahuanga; Kimberle C Chapin; Michael C Musgnug; Michael H Levi; Cynthia Shoemaker; Henrik Stender |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 41 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-07 Completed Date: 2003-05-02 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 889-91 Citation Subset: IM |
Affiliation:
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Applied Biosystems, Bedford, Boston , Massachusetts, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteriological Techniques Culture Media DNA Probes / diagnostic use In Situ Hybridization, Fluorescence / methods* Peptide Nucleic Acids / diagnostic use Staphylococcus aureus / genetics, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DNA Probes; 0/Peptide Nucleic Acids |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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