| Direct human T helper cell-induced B cell activation is not mediated by inositol lipid hydrolysis. | |
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MedLine Citation:
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PMID: 2831275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Ag-specific interaction between cloned allospecific human Th cells and class II MHC determinants on the surface of allogeneic B cells induces a significant fraction of resting B cells to express a B cell specific activation Ag BLAST-2 (CD23). On the other hand, cross-linking of B cell surface Ig R by Ag analogues does not lead to BLAST-2 expression. By utilizing the BLAST-2 induction assay as a positive control for efficient Th-B cell interaction, we have investigated the biochemical basis of human B cell activation mediated by Ag and Th cells. Our data demonstrate that ligands for sIg R, including F(ab')2 goat anti-human IgM and Staphylococcus aureus protein A, stimulate the metabolism of B cell membrane inositol lipids as assessed by: 1) increased [3H]inositol phosphates formation in myo-[3H]inositol-labeled B cells; 2) selective incorporation of [32P]orthophosphate into phosphatidic acid and phosphatidylinositol, but not into phosphatidylethanolamine or phosphatidylcholine; and 3) rapid increase in B cell cytoplasmic ionized Ca2+ concentration ([Ca2+]i). In contrast, direct Th-B cell interaction leads to high intensity BLAST-2 expression on the B cell surface but this response is not mediated by changes in inositol lipid metabolism or [Ca2+]i. Further, Th-B cell interaction does not affect the changes in B cell inositol lipid metabolism or [Ca2+]i triggered by sIg cross-linking. Taken together, our results suggest that Ag and Th cells induce different functional B cell responses by activating distinct second messenger systems within the B cell. |
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Authors:
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E K Chartash; A Imai; M C Gershengorn; M K Crow; S M Friedman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 140 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-04-20 Completed Date: 1988-04-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1974-81 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Hospital for Special Surgery, New York, NY 10021. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens
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immunology Antigens, Differentiation, B-Lymphocyte / biosynthesis*, immunology B-Lymphocytes / immunology* Calcium / metabolism Humans Hydrolysis Lymphocyte Activation Lymphocyte Cooperation* Membrane Lipids / metabolism* Phosphatidylinositols / metabolism* Receptors, Antigen, B-Cell / immunology T-Lymphocytes, Helper-Inducer / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AI-18263/AI/NIAID NIH HHS; AM-01523/AM/NIADDK NIH HHS; DK-33468/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Antigens, Differentiation, B-Lymphocyte; 0/Membrane Lipids; 0/Phosphatidylinositols; 0/Receptors, Antigen, B-Cell; 7440-70-2/Calcium |
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