Document Detail


Direct formation of proteo-liposomes by in vitro synthesis and cellular cytosolic delivery with connexin-expressing liposomes.
MedLine Citation:
PMID:  19423159     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Liposomes are widely utilized in molecular biology and medicine as drug carriers. Here we report a new liposome-cell interaction through connexins. Connexin 43 (Cx43)-containing liposomes were prepared by using cell-free transcription/translation systems with plasmids encoding Cx43 in the presence of liposome. The expressed membrane protein, Cx43, was directly constituted to the liposome membrane upon in vitro synthesis, leading to pure membrane protein-containing liposomes. The hydrophilic dye calcein was efficiently transferred from Cx43-expressing liposomes to cultured cells (Cx43 expressing). The transfer is significantly blocked in the presence of gap junction inhibitor (18beta-glycyrrhetinic acid) and in the case of the other type of connexin (Cx32)-expressing cell. The results show that calcein entered the cell through connexin-mediated pathway. Cx43 liposomes containing a soluble NEMO-binding domain peptide suppressed the intracellular signaling cascade IL-1beta-induced NF-kappaB activation and cyclooxygenase-2 expression in Cx43-expressing cells, confirming effective peptide transfer into the cell. This is a new method for direct cytosolic delivery of hydrophilic molecules.
Authors:
Makoto Kaneda; Shin-ichiro M Nomura; Shizuko Ichinose; Satoshi Kondo; Ken-ichi Nakahama; Kazunari Akiyoshi; Ikuo Morita
Related Documents :
18331509 - Low-frequency electromagnetic field effects on functional groups in human skin keratino...
19230649 - Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.
20480109 - Handheld mechanical cell lysis chip with ultra-sharp silicon nano-blade arrays for rapi...
20352759 - Thin film nano solar cells--from device optimization to upscaling.
16635569 - Microsystem for transfection of exogenous molecules with spatio-temporal control into a...
19199389 - Electroporation of cells in microfluidic droplets.
15914219 - Cellular uptake of avian leukosis virus subgroup b is mediated by clathrin.
22196899 - The role of hydrogels with tethered acetylcholine functionality on the adhesion and via...
7828599 - B-cell proliferation and induction of early g1-regulating proteins by epstein-barr viru...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-05-06
Journal Detail:
Title:  Biomaterials     Volume:  30     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-06-05     Completed Date:  2009-08-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3971-7     Citation Subset:  IM    
Affiliation:
Department of Cellular Physiological Chemistry, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents / pharmacology
Cell Line, Tumor
Connexin 43 / chemistry
Connexins / chemistry*
Drug Carriers / chemistry*,  metabolism
Fluoresceins / metabolism,  pharmacology
Gap Junctions / drug effects,  metabolism
Glycyrrhetinic Acid / analogs & derivatives,  pharmacology
Humans
Liposomes / chemistry*,  pharmacology
Microscopy, Immunoelectron
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Connexin 43; 0/Connexins; 0/Drug Carriers; 0/Fluoresceins; 0/Liposomes; 1449-05-4/18alpha-glycyrrhetinic acid; 1461-15-0/fluorexon; 471-53-4/Glycyrrhetinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Gender and grade-level comparisons in the structure of problem behaviors among adolescents.
Next Document:  Intracellular Ca(2+) stores modulate SOCCs and NMDA receptors via tyrosine kinases in rat hippocampa...