Document Detail

Direct extraction of specific pharmacophoric flavonoids from gingko leaves using a molecularly imprinted polymer for quercetin.
MedLine Citation:
PMID:  11762756     Owner:  NLM     Status:  MEDLINE    
A new straightforward approach to extract active components from Chinese traditional herb was reported by using a molecularly imprinted polymer (MIP) as the sorbent material. The MIP was prepared using quercetin, a typical active compound of the flavonoid family in gingko leaves as the template. Acrylamide was used as the functional monomer and two polar solvent tetrahydrofuran was used as the porogen. Afterwards, the polymer was evaluated by chromatographic analysis. It exhibited high selectivity for quercetin and good affinity to its structural analogues. Specific binding amount of quercetin on the MIP in the solvent methanol was 12 microg/g polymer by the cartridge test. The MIP cartridge could directly trap a specific class of compounds including quercetin and kaempferol from the hydrolyzate of gingko leaves. The result demonstrated the possibility of direct extraction of certain pharmacophoric constituents from herb by MIP technology. The compounds extracted were confirmed by time-of-flight mass spectrometry.
J Xie; L Zhu; H Luo; L Zhou; C Li; X Xu
Related Documents :
2071486 - Cyclothiazomycin, a novel polythiazole-containing peptide with renin inhibitory activit...
20403626 - Influence of methionine and dithioerythritol on sperm motility, lipid peroxidation and ...
19363516 - Identification of anti-asthmatic compounds in pericarpium citri reticulatae and evaluat...
19827026 - Antifungal activity of the aqueous extract of ilex paraguariensis against malassezia fu...
24062046 - Imatinib quantification in human serum for clinical purposes using high-performance liq...
23680846 - Electrocatalytic oxidation and selective determination of an opioid analgesic methadone...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatography. A     Volume:  934     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-12-12     Completed Date:  2002-05-02     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM    
Beida Yangshengtang Joint Laboratory for Natural Products, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, High Pressure Liquid / methods
Flavonoids / isolation & purification*
Ginkgo biloba / chemistry*
Plant Leaves / chemistry*
Polymers / chemistry*
Quercetin / chemistry*
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Reg. No./Substance:
0/Flavonoids; 0/Polymers; 117-39-5/Quercetin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Characterization of two genes encoding ferritin-like protein in Actinobacillus actinomycetemcomitans...
Next Document:  Strategies for in-capillary derivatization of amino acids in capillary electrophoresis using 1,2-nap...