Document Detail

Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification.
MedLine Citation:
PMID:  1476448     Owner:  NLM     Status:  MEDLINE    
In this report we describe a simple and rapid technique using DNA affinity columns that permits direct extraction of bacterial plasmids from a variety of foods for polymerase chain reaction amplification. The procedure was used to detect virulent enteroinvasive Escherichia coli in several artificially seeded matrices, including seafoods, greens, dairy products, enrichment media, and water. Polymerase inhibitors present in both foods and enrichment media were removed efficiently.
M R Andersen; C J Omiecinski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  58     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-02-01     Completed Date:  1993-02-01     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4080-2     Citation Subset:  IM    
Food and Drug Administration-Seattle District Office, Bothell, Washington 98041-3012.
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MeSH Terms
DNA, Bacterial / genetics,  isolation & purification
Escherichia coli / genetics*,  isolation & purification*,  pathogenicity
Escherichia coli Infections / prevention & control
Evaluation Studies as Topic
Food Microbiology*
Foodborne Diseases / prevention & control
Polymerase Chain Reaction / methods*
Restriction Mapping
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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