Document Detail

Direct examination of chemical kinetic laws by visual imagery. IV. Association processes between antigen- and fragmented antibody carrying fluorescent latex particles.
MedLine Citation:
PMID:  2706309     Owner:  NLM     Status:  MEDLINE    
The binary association process was further examined by direct visual observation using a fluorescence microscope connected to an image-processing system and by a spectrophotometric method. In the present paper, we used human serum albumin (HSA)- and fragmented anti-HSA antibody-carrying fluorescent latex particles in order to attain higher sensitivities and exclude complexities that might be caused by dissociation of the association product. The rate constant of the binary association process was estimated to be 6.0 x 10(8) M-1 s-1 at 30 degrees C and pH 7.6. The activation parameters of the association process were evaluated. The effects of pH and viscosity of the suspension on the association process were also examined.
H Kitano; C H Yan; Y Maeda; N Ise
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biopolymers     Volume:  28     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-26     Completed Date:  1989-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  693-702     Citation Subset:  IM    
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MeSH Terms
Antigen-Antibody Complex / analysis*
Serum Albumin / immunology
Spectrometry, Fluorescence / methods
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Latex; 0/Serum Albumin

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