Document Detail


Direct examination of chemical kinetic laws by visual imagery. IV. Association processes between antigen- and fragmented antibody carrying fluorescent latex particles.
MedLine Citation:
PMID:  2706309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The binary association process was further examined by direct visual observation using a fluorescence microscope connected to an image-processing system and by a spectrophotometric method. In the present paper, we used human serum albumin (HSA)- and fragmented anti-HSA antibody-carrying fluorescent latex particles in order to attain higher sensitivities and exclude complexities that might be caused by dissociation of the association product. The rate constant of the binary association process was estimated to be 6.0 x 10(8) M-1 s-1 at 30 degrees C and pH 7.6. The activation parameters of the association process were evaluated. The effects of pH and viscosity of the suspension on the association process were also examined.
Authors:
H Kitano; C H Yan; Y Maeda; N Ise
Related Documents :
15509889 - Optical techniques for imaging membrane topography.
18260609 - Noninvasive optical imaging of staphylococcus aureus bacterial infection in living mice...
24093439 - 3d super-resolution imaging with blinking quantum dots.
18800029 - In vivo visualization of albumin degradation in the proximal tubule.
12880339 - Spectroscopic approach for monitoring two-photon excited fluorescence resonance energy ...
24106059 - Growth of the human ilium: the anomalous sacroiliac junction.
22083889 - Cover image.
25368689 - A clinical case of fournier's gangrene: imaging ultrasound.
24595349 - Convection-reaction equation based magnetic resonance electrical properties tomography ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biopolymers     Volume:  28     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-26     Completed Date:  1989-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  693-702     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antigen-Antibody Complex / analysis*
Kinetics
Latex
Serum Albumin / immunology
Spectrometry, Fluorescence / methods
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Latex; 0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  In vivo degradation of polymers. I. Change of mechanical properties in polyethylene pacemaker lead i...
Next Document:  Proton nmr relaxation study of the dynamics of anthopleurin-A in solution.