Document Detail


Direct evidence for a chronic CD8+-T-cell-mediated immune reaction to tax within the muscle of a human T-cell leukemia/lymphoma virus type 1-infected patient with sporadic inclusion body myositis.
MedLine Citation:
PMID:  15367598     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human T-cell leukemia/lymphoma virus type 1 (HTLV-1) infection can lead to the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), concomitantly with or without other inflammatory disorders such as myositis. These pathologies are considered immune-mediated diseases, and it is assumed that migration within tissues of both HTLV-1-infected CD4(+) T cells and anti-HTLV-1 cytotoxic T cells represents a pivotal event. However, although HTLV-1-infected T cells were found in inflamed lesions, the antigenic specificity of coinfiltrated CD8(+) T cells remains to be determined. In this study, we performed both ex vivo and in situ analyses using muscle biopsies obtained from an HTLV-1-infected patient with HAM/TSP and sporadic inclusion body myositis. We found that both HTLV-1-infected CD4(+) T cells and CD8(+) T cells directed to the dominant Tax antigen can be amplified from muscle cell cultures. Moreover, we were able to detect in two successive muscle biopsies both tax mRNA-positive mononuclear cells and T cells recognized by the Tax11-19/HLA-A*02 tetramer and positive for perforin. These findings provide the first direct demonstration that anti-Tax cytotoxic T cells are chronically recruited within inflamed tissues of an HTLV-1 infected patient, which validates the cytotoxic immune reaction model for the pathogenesis of HTLV-1-associated inflammatory disease.
Authors:
Simona Ozden; Madeleine Cochet; Jacqueline Mikol; Antonio Teixeira; Antoine Gessain; Claudine Pique
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of virology     Volume:  78     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-09-15     Completed Date:  2004-10-21     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10320-7     Citation Subset:  IM    
Affiliation:
Unité d'Epidémiologie et Physiopathologie des Virus Oncogènes, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Amino Acid Sequence
Antigens, Viral / immunology
CD4-Positive T-Lymphocytes / virology
Cells, Cultured
Female
Gene Products, tax / immunology*
HLA-A Antigens / metabolism
HTLV-I Infections / immunology*
Human T-lymphotropic virus 1 / immunology*,  pathogenicity
Humans
Inclusion Bodies, Viral
Membrane Glycoproteins / analysis
Molecular Sequence Data
Muscles / pathology,  virology
Myositis / immunology*,  pathology,  virology
Paraparesis, Tropical Spastic / immunology,  virology
Perforin
Pore Forming Cytotoxic Proteins
RNA, Messenger / analysis
RNA, Viral / analysis
T-Lymphocytes, Cytotoxic / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Viral; 0/Gene Products, tax; 0/HLA-A Antigens; 0/Membrane Glycoproteins; 0/Pore Forming Cytotoxic Proteins; 0/RNA, Messenger; 0/RNA, Viral; 126465-35-8/Perforin
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