| Direct evaluation of stereoselectivity of cancer esterases by polyacrylamide gel electrophoresis coupled with activity staining with chiral naphthyl esters. | |
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MedLine Citation:
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PMID: 8594976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Both enantiomers of alpha-naphthyl 2-phenylpropanoate (PhPr(ONap)), N-acetylalaninate (AcAla(ONap)), N-methoxycarbonylalaninate (MocAla(ONap)), N-methoxycarbonylvalinate (MocVal(ONap)), N-acetylprolinate (AcPro(ONap)), and N-(trifluoroacetyl)prolinate (TfaPro(ONap)) were prepared and used with Fast Blue RR salt for activity staining of esterases separated by polyacrylamide gel electrophoresis. Comparison of the band thicknesses stained with each enantiomer indicates stereoselectivity of the major esterase in that band. Several esterases in normal rat liver, rat hepatoma-derived cells, and mouse B16 melanoma showed alteration or inversion of stereoselectivity by the substrate change from MocAla(ONap) to MocVal(ONap) or from AcPro(ONap) to TfaPro(ONap). The stereoselectivity of the major esterases for MocVal(ONap) was reversed between the murine liver and the B16 melanoma enzymes. The present staining with chiral naphthyl esters is very effective for surveying rapidly the stereoselectivity of animal tissue and cancer esterases. |
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Authors:
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Y Yamazaki; Y Kageyama; H Okuno |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical biochemistry Volume: 231 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1995 Nov |
Date Detail:
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Created Date: 1996-04-09 Completed Date: 1996-04-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 295-300 Citation Subset: IM |
Affiliation:
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National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electrophoresis, Polyacrylamide Gel* Esterases / analysis* Esters Evaluation Studies as Topic Female Liver Neoplasms / enzymology* Liver Neoplasms, Experimental / enzymology* Male Melanoma, Experimental / enzymology* Mice Mice, Inbred C57BL Naphthols* Protein Conformation Rats Rats, Wistar Staining and Labeling Stereoisomerism Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Naphthols; EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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