Document Detail


Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS.
MedLine Citation:
PMID:  25253534     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 μM were detected at a temporal resolution of 1 s.
Authors:
L J Friesen-Waldner; C N Wiens; T P Wade; K Thind; K P Sinclair; Y Hovav; J M Gomori; J Sosna; C A McKenzie; R Katz-Brull
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-9-25
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  -     ISSN:  1364-548X     ISO Abbreviation:  Chem. Commun. (Camb.)     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-9-25     Completed Date:  -     Revised Date:  2014-9-26    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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