Document Detail


Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica-MCM-41 matrix.
MedLine Citation:
PMID:  17107826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The direct electrochemistry of glucose oxidase (GOD) immobilized on a hexagonal mesoporous silica modified glassy carbon electrode was investigated. The adsorbed GOD displayed a pair of redox peaks with a formal potential of -417 mV in 0.1 M pH 6.1 phosphate buffer solution (PBS). The response showed a diffusion-controlled electrode process with a two-electron transfer coupled with a two-proton transfer reaction process. GOD immobilized on a hexagonal mesoporous silica retained its bioactivity and stability. In addition, the immobilized GOD could electrocatalyze the oxidation of glucose to gluconlactone by taking ferrocene monocarboxylic acid (FMCA) as a mediator in N(2) saturated solutions, indicating that the electrode may have the potential application in biosensors to analyze glucose. The sensor could exclude the interference of commonly coexisted uric acid, p-acetaminophenol and ascorbic acid and diagnose diabetes very fast and sensitively. This work demonstrated that the mesoporous silica provided a novel matrix for protein immobilization and the construction of biosensors.
Authors:
Z H Dai; J Ni; X H Huang; G F Lu; J C Bao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-30
Journal Detail:
Title:  Bioelectrochemistry (Amsterdam, Netherlands)     Volume:  70     ISSN:  1567-5394     ISO Abbreviation:  Bioelectrochemistry     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-06     Completed Date:  2007-06-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100953583     Medline TA:  Bioelectrochemistry     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  250-6     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Nanjing Normal University, Nanjing, PR China. daizhihuii@njnu.edu.cn <daizhihuii@njnu.edu.cn>
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / instrumentation,  methods*
Coated Materials, Biocompatible / chemistry
Electrochemistry / instrumentation,  methods*
Enzymes, Immobilized / chemistry
Glucose / analysis*,  chemistry*
Glucose Oxidase / chemistry*
Porosity
Reproducibility of Results
Sensitivity and Specificity
Silicon Dioxide / chemistry*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Enzymes, Immobilized; 50-99-7/Glucose; 7631-86-9/Silicon Dioxide; EC 1.1.3.4/Glucose Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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