| Direct and efficient cellular transformation of primary rat mesenchymal precursor cells by KSHV. | |
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MedLine Citation:
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PMID: 22293176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infections by viruses are associated with approximately 12% of human cancer. Kaposi's sarcoma-associated herpesvirus (KSHV) is causally linked to several malignancies commonly found in AIDS patients. The mechanism of KSHV-induced oncogenesis remains elusive, due in part to the lack of an adequate experimental system for cellular transformation of primary cells. Here, we report efficient infection and cellular transformation of primary rat embryonic metanephric mesenchymal precursor cells (MM cells) by KSHV. Cellular transformation occurred at as early as day 4 after infection and in nearly all infected cells. Transformed cells expressed hallmark vascular endothelial, lymphatic endothelial, and mesenchymal markers and efficiently induced tumors in nude mice. KSHV established latent infection in MM cells, and lytic induction resulted in low levels of detectable infectious virions despite robust expression of lytic genes. Most KSHV-induced tumor cells were in a latent state, although a few showed heterogeneous expression of lytic genes. This efficient system for KSHV cellular transformation of primary cells might facilitate the study of growth deregulation mechanisms resulting from KSHV infections. |
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Authors:
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Tiffany Jones; Fengchun Ye; Roble Bedolla; Yufei Huang; Jia Meng; Liwu Qian; Hongyi Pan; Fuchun Zhou; Rosalie Moody; Brent Wagner; Mazen Arar; Shou-Jiang Gao |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2012-02-01 |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 122 ISSN: 1558-8238 ISO Abbreviation: J. Clin. Invest. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-03-02 Completed Date: 2012-04-30 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: United States |
Other Details:
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Languages: eng Pagination: 1076-81 Citation Subset: AIM; IM |
Affiliation:
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Tumor Virology Program, Greehey Children’s Cancer Research Institute, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Line Cell Transformation, Viral Embryonic Stem Cells / cytology* Gene Expression Regulation* Herpesvirus 8, Human / metabolism* Human Umbilical Vein Endothelial Cells Humans Mesenchymal Stromal Cells / cytology* Mice Mice, Nude Microcirculation Open Reading Frames Rats Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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CA096512/CA/NCI NIH HHS; CA124332/CA/NCI NIH HHS; CA132637/CA/NCI NIH HHS; R01 CA096512-06/CA/NCI NIH HHS; R01 CA096512-07/CA/NCI NIH HHS; R01 CA096512-08/CA/NCI NIH HHS; R01 CA096512-09/CA/NCI NIH HHS; R01 CA124332-01A2/CA/NCI NIH HHS; R01 CA124332-02/CA/NCI NIH HHS; R01 CA124332-03/CA/NCI NIH HHS; R01 CA124332-04/CA/NCI NIH HHS; R01 CA124332-05/CA/NCI NIH HHS; R01 CA124332-06/CA/NCI NIH HHS; R01 CA132637-01A1/CA/NCI NIH HHS; R01 CA132637-02/CA/NCI NIH HHS; R01 CA132637-02S1/CA/NCI NIH HHS; R01 CA132637-03/CA/NCI NIH HHS; R01 CA132637-03S1/CA/NCI NIH HHS; R01 CA132637-04/CA/NCI NIH HHS; R01 CA132637-04S1/CA/NCI NIH HHS; R01 CA132637-05/CA/NCI NIH HHS; R01 CA132637-06/CA/NCI NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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