Document Detail


Direct effects of snowdrop lectin (GNA) on larvae of three aphid predators and fate of GNA after ingestion.
MedLine Citation:
PMID:  16620868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants genetically modified to express Galanthus nivalis agglutinin (GNA) have been found to confer partial resistance to homopteran pests. Laboratory experiments were conducted to investigate direct effects of GNA on larvae of three species of aphid predators that differ in their feeding and digestive physiology, i.e. Chrysoperla carnea, Adalia bipunctata and Coccinella septempunctata. Longevity of all three predator species was directly affected by GNA, when they were fed a sucrose solution containing 1% GNA. However, a difference in sensitivity towards GNA was observed when comparing the first and last larval stage of the three species. In vitro studies revealed that gut enzymes from none of the three species were able to break down GNA. In vivo feed-chase studies demonstrated accumulation of GNA in the larvae. After the larvae had been transferred to a diet devoid of GNA, the protein stayed present in the body of C. carnea, but decreased over time in both ladybirds. Binding studies showed that GNA binds to glycoproteins that can be found in the guts of larvae of all three predator species. Immunoassay by Western blotting of haemolymph samples only occasionally showed the presence of GNA. Fluorescence microscopy confirmed GNA accumulation in the midgut of C. carnea larvae. Implications of these findings for non-target risk assessment of GNA-transgenic crops are discussed.
Authors:
Petra A M Hogervorst; Natalie Ferry; Angharad M R Gatehouse; Felix L Wäckers; Jörg Romeis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-06
Journal Detail:
Title:  Journal of insect physiology     Volume:  52     ISSN:  0022-1910     ISO Abbreviation:  J. Insect Physiol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-29     Completed Date:  2006-09-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985080R     Medline TA:  J Insect Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  614-24     Citation Subset:  IM    
Affiliation:
Agroscope FAL Reckenholz, Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zurich, Switzerland. petra.hogervorst@fal.admin.ch
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MeSH Terms
Descriptor/Qualifier:
Animals
Aphids
Beetles / drug effects*
Feeding Behavior / drug effects
Galanthus / chemistry*
Gastrointestinal Tract / drug effects
Hemolymph / chemistry
Immunohistochemistry
Insect Proteins / drug effects
Insecticides / analysis*
Insects / drug effects
Larva / drug effects*
Longevity / drug effects
Mannose-Binding Lectins / pharmacokinetics,  pharmacology*
Microscopy, Fluorescence
Pest Control, Biological
Plant Lectins / pharmacokinetics,  pharmacology*
Predatory Behavior
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/Insecticides; 0/Mannose-Binding Lectins; 0/Plant Lectins; 0/snowdrop lectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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