Document Detail


Direct effects of nutrients, acetylcholine, CCK, and insulin on ghrelin release from the isolated stomachs of rats.
MedLine Citation:
PMID:  19463754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ghrelin is a powerful orexigenic peptide predominantly secreted by the stomach. Blood concentration of ghrelin increases before meals and fall postprandial. Its regulation appears to be influenced by the type of macronutrient ingested, the vagus nerve stimulation and by other post-meal stimulated hormonal factors. However, the direct role of nutrients (amino acids or lipids), neuronal (vagal neurotransmitter acetylcholine) and satiety-inducing factor such as CCK are not known. To study this we applied amino acids, lipids, acetylcholine and CCK via vascular perfusion to the isolated stomachs and found that amino acids significantly reduced ghrelin release from the isolated stomach by approximately approximately 30% vs. the control while lipids (10% intralipid) had no affect. Acetylcholine (1 microM) increased ghrelin release from the stomach by approximately 37% whereas insulin (10nM) decreased it by approximately 30% vs. the control. Interestingly, CCK (100 nM) potently increased ghrelin release by approximately 200% vs. the control. Therefore it appears that ghrelin secretion from the stomach is under direct influence of amino acids, neurotransmitter acetylcholine and hormones such as insulin and CCK.
Authors:
Yogendra B Shrestha; Kathie Wickwire; Silvia Q Giraudo
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-02-12
Journal Detail:
Title:  Peptides     Volume:  30     ISSN:  1873-5169     ISO Abbreviation:  Peptides     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-09-01     Revised Date:  2011-09-26    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1187-91     Citation Subset:  IM    
Affiliation:
Department of Foods and Nutrition, University of Georgia, Athens, GA 30602, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology*
Amino Acids / pharmacology*
Animals
Cholecystokinin / pharmacology*
Gastric Mucosa / drug effects,  secretion*
Ghrelin / drug effects*,  metabolism,  secretion
Insulin / pharmacology*
Lipids / pharmacology*
Male
Rats
Rats, Sprague-Dawley
Stomach / drug effects,  metabolism,  surgery
Grant Support
ID/Acronym/Agency:
NIDDK-DK 59836//PHS HHS; R01 DK059836-01/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Ghrelin; 0/Lipids; 11061-68-0/Insulin; 51-84-3/Acetylcholine; 9011-97-6/Cholecystokinin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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