Document Detail


Direct effect of halothane and isoflurane on the function of the sarcoplasmic reticulum in intact rabbit atria.
MedLine Citation:
PMID:  2321786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The negative inotropic effect of halothane and isoflurane on potentiated-state contractions of isolated rabbit atria in a normal Ca2+ (2.5 mM) medium was compared with the force depression in low Ca2+ media without an anesthetic. When this comparison was made in the presence of 1 microM ryanodine so that the force of contraction was dependent only upon transsarcolemmal Ca2+ influx with no Ca2+ contribution from the sarcoplasmic reticulum (SR), the force of contraction was depressed equally by 0.6% halothane in a normal Ca2+ medium and by a 1.5 mM Ca2+ medium without the anesthetic. Similarly, 1.0% halothane or 1.5% isoflurane and a 1.0 mM Ca2+ medium were equally depressant as were 2.4% isoflurane and a 0.5 mM Ca2+ medium. In the absence of ryanodine, where the atrial contractile activity is largely dependent on Ca2+ released from the SR, 0.6% halothane in the normal Ca2+ medium depressed contractile force by 32%, whereas the force was depressed by only 16% in the 1.5 mM Ca2+ medium without the anesthetic. Similar results were obtained when the effects of 1.0% halothane and of 1.0 mM Ca2+ were compared. In contrast, the force of contraction measured in the absence of ryanodine was not at all inhibited by 1.5% isoflurane and minimally (11%) inhibited by 2.4% isoflurane. Consequently, the force depression by isoflurane was less than that found in the low Ca2+ media.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H Komai; B F Rusy
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Anesthesiology     Volume:  72     ISSN:  0003-3022     ISO Abbreviation:  Anesthesiology     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-02     Completed Date:  1990-05-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1300217     Medline TA:  Anesthesiology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  694-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Anesthesiology, University of Wisconsin, Madison 53792.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium / pharmacology
Culture Media
Halothane / pharmacology*
Heart / physiology*
Heart Atria
Isoflurane / pharmacology*
Myocardial Contraction / drug effects
Myocardium / ultrastructure
Osmolar Concentration
Rabbits
Ryanodine / pharmacology
Sarcoplasmic Reticulum / physiology*
Grant Support
ID/Acronym/Agency:
GM29527/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 151-67-7/Halothane; 15662-33-6/Ryanodine; 26675-46-7/Isoflurane; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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