| Direct effect of gossypol on TR-ST cells: perturbation of rhodamine 123 accumulation in mitochondria. | |
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MedLine Citation:
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PMID: 6518226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Gossypol has deleterious effects directly on TR-ST cells originating from a rat testicular tumor. Exposure of TR-ST cells to gossypol (5 micrograms/ml) decreases their rate of protein synthesis approximately 30% within 1 h and 65% by greater than 10 h, causes intracellular vacuolation, changes cell shape from cobblestone to a rounded conformation and inhibits cell proliferation. Yet, these gossypol-treated cells remain viable, as assessed by their ability to hydrolyze fluorescein diacetate. Gossypol also perturbs mitochondrial transmembrane potential in TR-ST cells, as demonstrated by marked changes in rhodamine 123 staining. Mitochondria of control TR-ST cells avidly accumulate rhodamine 123, but those in cells exposed to gossypol (greater than or equal to 5 micrograms/ml) for greater than 1 h fail to sequester the fluorochrome. Instead, the cell cytoplasm shows a light and diffuse staining with rhodamine 123. Rat spermatozoa show a similar response. Conversely, at concentrations of 20 micrograms/ml, gossypol has minimal effects on rhodamine 123 accumulation by primary cultures of hepatocytes and by rat spermatogenic cells, including primary spermatocytes and spermatids (Steps 1-12). Moreover, TR-ST cells exhibit reduced mitochondrial staining with gossypol at an ED50 of 7.6 micrograms/ml, while those for the nontesticular Rat-1, AnAn, 3T3 and PtK2 cell lines are 13.1, 21.5, 28.5 and 26.4 micrograms/ml, respectively. |
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Authors:
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N Tanphaichitr; L B Chen; A R Bellvé |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biology of reproduction Volume: 31 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1985-03-14 Completed Date: 1985-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1049-60 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cell Line Dose-Response Relationship, Drug Fluorescent Dyes Gossypol / pharmacology* Kinetics Male Membrane Potentials / drug effects Mitochondria / drug effects, physiology* Protein Biosynthesis Rats Rhodamine 123 Rhodamines / metabolism* Spermatozoa / drug effects, physiology, ultrastructure Testicular Neoplasms / pathology, physiopathology* Xanthenes / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Rhodamines; 0/Xanthenes; 303-45-7/Gossypol; 62669-70-9/Rhodamine 123 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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