| Direct differentiation of hepatic cells from human induced pluripotent stem cells using a limited number of cytokines. | |
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MedLine Citation:
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PMID: 21484132 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: Development of improved protocols for differentiating induced pluripotent stem (iPS) cells into hepatic cells is an important step toward their use in the field of hepatology. Specifically, the number of different cytokines should be reduced to limit undesired effects and to reduce the cost of the process. In this report, we describe a simple method for directing human iPS cells to differentiate into hepatic cells using only two cytokines and a short incubation time. METHODS: A two-step protocol for differentiating iPS cells into hepatic cells was developed. A high dose of activin A was applied for 3 days to induce definitive endoderm formation. Subsequently, cells were treated with hepatocyte growth factor (HGF) for 5 days to generate hepatic cells. Differentiation was confirmed by immunostaining for differentiation markers. Albumin mRNA levels in differentiated hepatic cells generated using a previously tested three-step protocol that uses activin A, fibroblast growth factor (FGF)/bone morphogenetic protein (BMP), and HGF, and our new protocol were compared to determine the efficiency of differentiation. RESULTS: Our two-step protocol induced the differentiation of iPS cells into hepatic cells and required a shorter differentiation period than the previous three-step protocol. The differentiation efficiencies of the two protocols were comparable and the induced hepatic cells were functional. CONCLUSIONS: Developing efficient induction and culture methods to generate more highly matured hepatocytes is essential for regenerative cell-based therapies. Our protocol provides a simple, cost-effective, and time-saving approach for generating hepatic cells from iPS cells. |
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Authors:
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Akemi Takata; Motoyuki Otsuka; Tomomi Kogiso; Kentaro Kojima; Takeshi Yoshikawa; Ryosuke Tateishi; Naoya Kato; Shuichiro Shiina; Haruhiko Yoshida; Masao Omata; Kazuhiko Koike |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-2-6 |
Journal Detail:
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Title: Hepatology international Volume: - ISSN: 1936-0541 ISO Abbreviation: - Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-4-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101304009 Medline TA: Hepatol Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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