Document Detail


Direct determination of uric acid in serum by a fluorometric-enzymatic method based on uricase.
MedLine Citation:
PMID:  18968307     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The present paper describes a method for the fluorometric determination of uric acid in blood serum by its reaction with uricase (UOx). The procedure is based on the changes in fluorescence that take place during the enzymatic reaction of UOx with uric acid when the solution is excited at 287 nm and the emission is measured at 330 nm. A mathematical model which relates the analytical signal to the analyte concentration was developed and the model also served to obtain some of the thermodynamic constants of the system (the Michaelis constant and the turnover number). The optimum reaction conditions and its analytical characteristics were studied, linear response range (3x10(-5)-6x10(-4) M) and reproducibility (4%, n=7). The method was applied to the determination of uric acid in three blood serum samples. The results were compared with those obtained by a commercial clinical analyzer and no systematic errors were observed.
Authors:
J Galbán; Y Andreu; M J Almenara; S de Marcos; J R Castillo
Related Documents :
8890397 - Uric acid stones.
22021057 - Embryo and larva development in dentex dentex, a marine pelagophil teleost: an endeavor...
7869917 - Hyperuricemia in patients with hyperthyroidism due to graves' disease.
15164327 - Effect of sauna bathing and beer ingestion on plasma concentrations of purine bases.
7800117 - Brain, liver, and adipose tissue erucic and very long chain fatty acid levels in adreno...
1176597 - Biotypes of proteus rettgeri.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  54     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  847-54     Citation Subset:  -    
Affiliation:
GEAS, Analytical Spect. and Sensors Group, Department of Analytical Chemistry, Science Faculty, University of Zaragoza, Zaragoza E-50009, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of acid rain on soil humic compounds.
Next Document:  Direct determination of trace amounts of sodium in water-soluble organic pharmaceuticals by microwav...