Document Detail


Direct determination of polymyxin B sulfate using resonance Rayleigh scattering and resonance non-linear scattering methods with hexatungstate.
MedLine Citation:
PMID:  23299991     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
At pH 1.3-1.6, tungstate WO(4) (2-) , can be converted to hexatungstate W(6) O(19) (2-) , which can react with positively charged polymyxin B sulfate (PMB) to result in enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, including second order scattering and frequency doubling scattering. Linear relationships can be established between enhanced scattering intensity and PMB concentration. The detection limits (3σ) were 5.5 ng/mL (RRS), 10.1 ng/mL (second order scattering) and 34.6 ng/mL (frequency doubling scattering). The optimum reaction conditions, influencing factors and related analytical properties were tested. The interaction mechanism was investigated via absorption spectrum, circular dichroism spectra and atomic force microscopy imaging. The basis of scattering enhancement is discussed. PMB in eardrops, human serum and urine, were quantified satisfactorily by RRS. Copyright © 2013 John Wiley & Sons, Ltd.
Authors:
Ling Kong; Zhongfang Liu; Shaopu Liu; Limin Wu; Fengling Tian
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  -     ISSN:  1522-7243     ISO Abbreviation:  Luminescence     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Affiliation:
School of Chemistry, Chemical Engineering, Key Laboratory of Luminescence, Real-time Analysis of the Ministry of Education, Southwest University, Chongqing, China.
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