| Direct determination of mitoxantrone in plasma by high performance liquid chromatography using an automatic precolumn-switching system as sample clean-up procedure. | |
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MedLine Citation:
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PMID: 8130632 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A high-performance liquid chromatography method which uses direct injection and a column-switching valve for determination of mitoxantrone in plasma is described. After addition of internal standard, plasma was deproteinized by adding 5-sulphosalicylic acid reagent. The supernatant was injected onto an enrichment precolumn flushed with washing solvent (methanol and water 5:95). Absorbed mitoxantrone was backflushed from the precolumn into an analytical column C18 Nucleosil 250 x 4 mm with a gradient elution (solvent A, ammonium formate buffer 1.6 M, pH 4.3; solvent B, acetonitrile and water 40:60; linear gradient from 45 to 55% of B for 30 min was programmed) at a flow rate of 1.3 mL/min. Detection was carried out at 665 nm. This method showed obvious advantages over conventional extraction procedures in terms of speed and facility of sample handling. |
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Authors:
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J Catalin; A F Peloux; F Coloma; B Payet; B Lacarelle; J P Cano; S Just-Landi |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomedical chromatography : BMC Volume: 8 ISSN: 0269-3879 ISO Abbreviation: Biomed. Chromatogr. Publication Date: 1994 Jan-Feb |
Date Detail:
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Created Date: 1994-04-21 Completed Date: 1994-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8610241 Medline TA: Biomed Chromatogr Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 37-41 Citation Subset: IM |
Affiliation:
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Laboratoire de Toxicocinétique et de Pharmacocinétique Faculté de Pharmacie, Marseille, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, High Pressure Liquid
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methods* Humans Mitoxantrone / blood* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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65271-80-9/Mitoxantrone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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