| Direct determination of benzodiazepines in biological fluids by restricted-access solid-phase microextraction. | |
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MedLine Citation:
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PMID: 11924967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A biocompatible solid-phase microextraction (SPME) fiber was prepared using an alkyl-diol-silica (ADS) restricted-access material as the SPME coating. The ADS-SPME fiber was able to simultaneously fractionate the protein component from a biological sample, while directly extracting several benzodiazepines, overcoming the present disadvantages of direct sampling in biological matrixes by SPME. The fiber was interfaced with an HPLC-UV system, and an isocratic mobile phase was used to desorb, separate, and quantify the extracted compounds. The calculated clonazepam, oxazepam, temazepam, nordazepam, and diazepam detection limits were 600, 750, 333, 100, and 46 ng/mL in urine, respectively. The method was confirmed to be linear over the range of 500-50000 ng/mL with an average linear coefficient (R2) value of 0.9918. The injection repeatability and intraassay precision of the method were evaluated over 10 injections, resulting in a RSD of approximately 6%. The ADS-SPME fiber was robust and simple to use, providing many direct extractions and subsequent determination of benzodiazepines in biological fluids. |
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Authors:
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Wayne M Mullett; Janusz Pawliszyn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Analytical chemistry Volume: 74 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-04-01 Completed Date: 2002-04-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1081-7 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Waterloo, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Anxiety Agents
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urine* Benzodiazepines Chromatography, High Pressure Liquid Humans Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Anti-Anxiety Agents; 12794-10-4/Benzodiazepines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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