Document Detail

Direct determination of benzodiazepines in biological fluids by restricted-access solid-phase microextraction.
MedLine Citation:
PMID:  11924967     Owner:  NLM     Status:  MEDLINE    
A biocompatible solid-phase microextraction (SPME) fiber was prepared using an alkyl-diol-silica (ADS) restricted-access material as the SPME coating. The ADS-SPME fiber was able to simultaneously fractionate the protein component from a biological sample, while directly extracting several benzodiazepines, overcoming the present disadvantages of direct sampling in biological matrixes by SPME. The fiber was interfaced with an HPLC-UV system, and an isocratic mobile phase was used to desorb, separate, and quantify the extracted compounds. The calculated clonazepam, oxazepam, temazepam, nordazepam, and diazepam detection limits were 600, 750, 333, 100, and 46 ng/mL in urine, respectively. The method was confirmed to be linear over the range of 500-50000 ng/mL with an average linear coefficient (R2) value of 0.9918. The injection repeatability and intraassay precision of the method were evaluated over 10 injections, resulting in a RSD of approximately 6%. The ADS-SPME fiber was robust and simple to use, providing many direct extractions and subsequent determination of benzodiazepines in biological fluids.
Wayne M Mullett; Janusz Pawliszyn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  74     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-01     Completed Date:  2002-04-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1081-7     Citation Subset:  IM    
Department of Chemistry, University of Waterloo, Ontario, Canada.
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MeSH Terms
Anti-Anxiety Agents / urine*
Chromatography, High Pressure Liquid
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Anti-Anxiety Agents; 12794-10-4/Benzodiazepines

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