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Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts.
MedLine Citation:
PMID:  25385968     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
To directly demonstrate the involvement of chloroplasts in the rapid light-induced potential change we removed chloroplasts by centrifuging tonoplast-free cells of Chara australis. Chloroplast-free cells showed no signs of a potential change on illumination. When a chloroplast-free Chara cell was perfused with a suspension of chloroplasts isolated from other cells of Chara australis, it regained its ability to generate the rapid potential change induced by light. Chloroplasts were isolated from spinach leaves by a method which preserves the chloroplast envelope; thus ensuring the ability to assimilate CO2. When these chloroplasts were introduced into a transparent Chara cell containing no chloroplasts, normal rapid hyperpolarization was induced on illumination. When spinach chloroplasts were treated with a medium of very low osmotic potential, no O2 evolution was detected, but even with these chloroplasts rapid light-induced hyperpolarization was observed. We concluded that chloroplasts are essential for the rapid light-induced potential change and that chloroplasts can be replaced with those of another species, so far as the light-induced potential change is concerned.
Authors:
M Tazawa; T Shimmen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant & cell physiology     Volume:  21     ISSN:  0032-0781     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  1980 Dec 
Date Detail:
Created Date:  2014-11-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1527-34     Citation Subset:  -    
Copyright Information:
Copyright © 1980. The Japanese Society of Plant Physiologists.
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