| Direct conversion of C. elegans germ cells into specific neuron types. | |
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MedLine Citation:
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PMID: 21148348 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of transcription factors to directly reprogram the identity of cell types is usually restricted and is defined by cellular context. Through the ectopic expression of single Caenorhabditis elegans transcription factors, we found that the identity of mitotic germ cells can be directly converted into that of specific neuron types: glutamatergic, cholinergic, or GABAergic. This reprogramming event requires the removal of the histone chaperone LIN-53 (RbAp46/48 in humans), a component of several histone remodeling and modifying complexes, and this removal can be mimicked by chemical inhibition of histone deacetylases. Our findings illustrate the ability of germ cells to be directly converted into individual, terminally differentiated neuron types and demonstrate that a specific chromatin factor provides a barrier for cellular reprogramming. |
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Authors:
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Baris Tursun; Tulsi Patel; Paschalis Kratsios; Oliver Hobert |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-12-09 |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 331 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-21 Completed Date: 2011-02-08 Revised Date: 2012-01-31 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 304-8 Citation Subset: IM |
Affiliation:
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Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University Medical Center, New York, NY 10032, USA. bt2189@columbia.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caenorhabditis elegans / cytology*, growth & development, metabolism Caenorhabditis elegans Proteins / genetics, metabolism*, physiology* Cell Differentiation* Chromatin / metabolism Gene Expression Germ Cells / cytology*, metabolism Glutamic Acid / metabolism Histone Deacetylase Inhibitors / pharmacology Histone Deacetylases / metabolism Histones / metabolism Homeodomain Proteins / genetics, metabolism Mitosis Motor Neurons / cytology, metabolism Muscle Cells / cytology, metabolism Neurogenesis* Neurons / cytology*, metabolism Nuclear Proteins / genetics, metabolism RNA Interference Repressor Proteins / genetics, physiology* Sensory Receptor Cells / cytology, metabolism Totipotent Stem Cells / cytology Transcription Factors / genetics, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS039996-12/NS/NINDS NIH HHS; R01 NS050266-09/NS/NINDS NIH HHS; R01NS039996-05/NS/NINDS NIH HHS; R01NS050266-03/NS/NINDS NIH HHS; //Howard Hughes Medical Institute |
| Chemical | |
Reg. No./Substance:
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0/CHE-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/Chromatin; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Homeodomain Proteins; 0/LIN-53 protein, C elegans; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/unc-3 protein, C elegans; 0/unc-30 protein, C elegans; 56-86-0/Glutamic Acid; EC 3.5.1.98/Histone Deacetylases |
| Comments/Corrections | |
Comment In:
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Nat Methods. 2011 Feb;8(2):112
[PMID:
21355125
]
Science. 2011 Jan 21;331(6015):292-3 [PMID: 21252336 ] Nat Rev Neurosci. 2011 Feb;12(2):60 [PMID: 21309088 ] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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