Document Detail


Direct conversion of C. elegans germ cells into specific neuron types.
MedLine Citation:
PMID:  21148348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of transcription factors to directly reprogram the identity of cell types is usually restricted and is defined by cellular context. Through the ectopic expression of single Caenorhabditis elegans transcription factors, we found that the identity of mitotic germ cells can be directly converted into that of specific neuron types: glutamatergic, cholinergic, or GABAergic. This reprogramming event requires the removal of the histone chaperone LIN-53 (RbAp46/48 in humans), a component of several histone remodeling and modifying complexes, and this removal can be mimicked by chemical inhibition of histone deacetylases. Our findings illustrate the ability of germ cells to be directly converted into individual, terminally differentiated neuron types and demonstrate that a specific chromatin factor provides a barrier for cellular reprogramming.
Authors:
Baris Tursun; Tulsi Patel; Paschalis Kratsios; Oliver Hobert
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-12-09
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  331     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-21     Completed Date:  2011-02-08     Revised Date:  2014-09-13    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  304-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / cytology*,  growth & development,  metabolism
Caenorhabditis elegans Proteins / genetics,  metabolism*,  physiology*
Cell Differentiation*
Chromatin / metabolism
Gene Expression
Germ Cells / cytology*,  metabolism
Glutamic Acid / metabolism
Histone Deacetylase Inhibitors / pharmacology
Histone Deacetylases / metabolism
Histones / metabolism
Homeodomain Proteins / genetics,  metabolism
Mitosis
Motor Neurons / cytology,  metabolism
Muscle Cells / cytology,  metabolism
Neurogenesis*
Neurons / cytology*,  metabolism
Nuclear Proteins / genetics,  metabolism
RNA Interference
Repressor Proteins / genetics,  physiology*
Sensory Receptor Cells / cytology,  metabolism
Totipotent Stem Cells / cytology
Transcription Factors / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 NS039996/NS/NINDS NIH HHS; R01 NS039996-12/NS/NINDS NIH HHS; R01 NS050266/NS/NINDS NIH HHS; R01 NS050266-09/NS/NINDS NIH HHS; R01NS039996-05/NS/NINDS NIH HHS; R01NS050266-03/NS/NINDS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/CHE-1 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/Chromatin; 0/Histone Deacetylase Inhibitors; 0/Histones; 0/Homeodomain Proteins; 0/LIN-53 protein, C elegans; 0/Nuclear Proteins; 0/Repressor Proteins; 0/Transcription Factors; 0/unc-3 protein, C elegans; 0/unc-30 protein, C elegans; 3KX376GY7L/Glutamic Acid; EC 3.5.1.98/Histone Deacetylases
Comments/Corrections
Comment In:
Science. 2011 Jan 21;331(6015):292-3   [PMID:  21252336 ]
Nat Methods. 2011 Feb;8(2):112   [PMID:  21355125 ]
Nat Rev Neurosci. 2011 Feb;12(2):60   [PMID:  21309088 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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