| Direct contact with mesenchymal stromal cells affects migratory behavior and gene expression profile of CD133+ hematopoietic stem cells during ex vivo expansion. | |
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MedLine Citation:
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PMID: 19216019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the impact of direct contact between mesenchymal stromal cells (MSCs) and CD133(+) hematopoietic stem cells in terms of expansion potential, differentiation, migratory capacity, and gene expression profile. MATERIALS AND METHODS: CD133(+)-purified hematopoietic progenitor cells were cultured for 7 days on subconfluent MSCs supplemented with growth-factor-containing medium. After ex vivo expansion, nonadherent and adherent cells were collected and analyzed separately. RESULTS: The adherent cell population was less differentiated than the nonadherent fraction. CXCR4 was upregulated in the adherent fraction, which was associated with a higher migration capacity toward a stromal cell-derived factor-1 gradient. Colony-forming unit granulocyte-macrophage and long-term culture-initiation cell assays demonstrated a higher clonogenicity and repopulating capacity of the adherent fraction. Genes involved in adhesion, cell-cycle control, motility, and self-renewal were more highly expressed in the adherent fraction. CONCLUSION: Adhesion and direct cell-to-cell contact with an MSC feeder layer supports ex vivo expansion, migratory potential, and stemness of CD133(+) hematopoietic progenitor cells. |
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Authors:
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Nael Alakel; Duohui Jing; Katrin Muller; Martin Bornhauser; Gerhard Ehninger; Rainer Ordemann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-12 |
Journal Detail:
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Title: Experimental hematology Volume: 37 ISSN: 1873-2399 ISO Abbreviation: Exp. Hematol. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-23 Completed Date: 2009-04-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 504-13 Citation Subset: IM |
Affiliation:
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Medizinische Klinik und Poliklinik I, University Hospital Carl-Gustav-Carus, Dresden, Germany. nael.alakel@uniklinikum-dresden.de |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism* Blotting, Western Cell Adhesion Cell Division / physiology Cell Movement / physiology* Flow Cytometry Gene Expression Profiling* Gene Expression Regulation* Glycoproteins / metabolism* Hematopoietic Stem Cells* / cytology, metabolism Humans Mesenchymal Stem Cells / cytology, metabolism* Peptides / metabolism* Stromal Cells / cytology, metabolism* Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides |
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