Document Detail


Direct contact with mesenchymal stromal cells affects migratory behavior and gene expression profile of CD133+ hematopoietic stem cells during ex vivo expansion.
MedLine Citation:
PMID:  19216019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the impact of direct contact between mesenchymal stromal cells (MSCs) and CD133(+) hematopoietic stem cells in terms of expansion potential, differentiation, migratory capacity, and gene expression profile. MATERIALS AND METHODS: CD133(+)-purified hematopoietic progenitor cells were cultured for 7 days on subconfluent MSCs supplemented with growth-factor-containing medium. After ex vivo expansion, nonadherent and adherent cells were collected and analyzed separately. RESULTS: The adherent cell population was less differentiated than the nonadherent fraction. CXCR4 was upregulated in the adherent fraction, which was associated with a higher migration capacity toward a stromal cell-derived factor-1 gradient. Colony-forming unit granulocyte-macrophage and long-term culture-initiation cell assays demonstrated a higher clonogenicity and repopulating capacity of the adherent fraction. Genes involved in adhesion, cell-cycle control, motility, and self-renewal were more highly expressed in the adherent fraction. CONCLUSION: Adhesion and direct cell-to-cell contact with an MSC feeder layer supports ex vivo expansion, migratory potential, and stemness of CD133(+) hematopoietic progenitor cells.
Authors:
Nael Alakel; Duohui Jing; Katrin Muller; Martin Bornhauser; Gerhard Ehninger; Rainer Ordemann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-12
Journal Detail:
Title:  Experimental hematology     Volume:  37     ISSN:  1873-2399     ISO Abbreviation:  Exp. Hematol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-23     Completed Date:  2009-04-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  504-13     Citation Subset:  IM    
Affiliation:
Medizinische Klinik und Poliklinik I, University Hospital Carl-Gustav-Carus, Dresden, Germany. nael.alakel@uniklinikum-dresden.de
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / metabolism*
Blotting, Western
Cell Adhesion
Cell Division / physiology
Cell Movement / physiology*
Flow Cytometry
Gene Expression Profiling*
Gene Expression Regulation*
Glycoproteins / metabolism*
Hematopoietic Stem Cells* / cytology,  metabolism
Humans
Mesenchymal Stem Cells / cytology,  metabolism*
Peptides / metabolism*
Stromal Cells / cytology,  metabolism*
Surface Properties
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Glycoproteins; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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