Document Detail


Direct comparison of progenitor cells derived from adipose, muscle, and bone marrow from wild-type or craniosynostotic rabbits.
MedLine Citation:
PMID:  20871482     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Reports have identified cells capable of osteogenic differentiation in bone marrow, muscle, and adipose tissues, but there are few direct comparisons of these different cell types. Also, few have investigated the potential connection between a tissue-specific abnormality and cells derived from seemingly unrelated tissues. In this article, the authors compare cells isolated from wild-type rabbits or rabbits with nonsyndromic craniosynostosis, defined as the premature fusion of one or more of the cranial sutures.
METHODS: Cells were derived from bone marrow, adipose, and muscle of 10-day-old wild-type rabbits (n = 17) or from age-matched rabbits with familial nonsyndromic craniosynostosis (n = 18). Cells were stimulated with bone morphogenetic protein-4 (BMP4), and alkaline phosphatase expression and cell proliferation were assessed.
RESULTS: In wild-type rabbits, cells derived from muscle had more alkaline phosphatase activity than cells derived from either adipose or bone marrow. The cells derived from craniosynostotic rabbit bone marrow and muscle were significantly more osteogenic than those derived from wild-type rabbits. Adipose-derived cells demonstrated no significant differences. Although muscle-derived cells were most osteogenic in wild-type rabbits, bone marrow-derived cells were most osteogenic in craniosynostotic rabbits.
CONCLUSIONS: These results suggest that cells from different tissues have different potentials for differentiation. Furthermore, cells derived from rabbits with craniosynostosis were different from cells from wild-type rabbits. Interestingly, cells derived from the craniosynostotic rabbits were not uniformly more responsive compared with wild-type cells, suggesting that specific tissue-derived cells may react differently in individuals with craniosynostosis.
Authors:
Gregory M Cooper; Emily L Durham; James J Cray; Michael R Bykowski; Gary E DeCesare; Melissa A Smalley; Mark P Mooney; Phil G Campbell; Joseph E Losee
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  127     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-04     Completed Date:  2011-02-03     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  88-97     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. greg.cooper@chp.edu
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology*
Alkaline Phosphatase / analysis
Animals
Bone Marrow Cells / cytology*
Bone Morphogenetic Protein 4 / pharmacology
Cell Division
Cell Separation / methods
Craniosynostoses / pathology*
Muscles / cytology*
Rabbits
Stem Cell Transplantation*
Stem Cells / physiology*
Tissue and Organ Harvesting / methods
Grant Support
ID/Acronym/Agency:
1-R01-DE019430-02/DE/NIDCR NIH HHS; R01 DE019430/DE/NIDCR NIH HHS; R01 DE019430-02/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/BMP4 protein, human; 0/Bone Morphogenetic Protein 4; EC 3.1.3.1/Alkaline Phosphatase
Comments/Corrections

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