Document Detail

Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum.
MedLine Citation:
PMID:  9365388     Owner:  NLM     Status:  MEDLINE    
A clinical need exists for a sensitive and specific assay for the quantitation of the bone isoform of alkaline phosphatase in serum. The majority of methods do not meet this requirement; however, the recent development of immunoassays for this isoform may provide a solution. In a detailed evaluation of two immunoassays, we found a degree of imprecision that enables the discrimination of changes within the reference range. The cross-reactivity of the liver isoform was found to be between 7.1% and 12.7% when two different methods of assessment were used. The comparison of results with an electrophoretic procedure showed that the immunocapture method recovered less of the bone isoform in samples from children than in samples from patients with Paget disease; no such difference was found with the immunometric method. This suggests that the immunocapture antibody may discriminate between different bone isoforms in children whereas the immunometric assay does not.
C P Price; T P Milligan; C Darte
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  43     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1997-12-08     Completed Date:  1997-12-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2052-7     Citation Subset:  IM    
Department of Clinical Biochemistry, st. Bartholomew's, London, UK.
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MeSH Terms
Alkaline Phosphatase / blood*
Bone and Bones / enzymology*
Clinical Enzyme Tests
Cross Reactions
Immunoassay / methods
Isoenzymes / blood*
Liver / enzymology
Liver Diseases / diagnosis
Osteitis Deformans / diagnosis
Reproducibility of Results
Reg. No./Substance:
0/Isoenzymes; EC Phosphatase

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