Document Detail


Direct comparison of the BD ProbeTec ET system with in-house LightCycler PCR assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae from clinical specimens.
MedLine Citation:
PMID:  15583309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The commercial BD ProbeTec ET (BDPT) system for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis from clinical specimens was compared with our in-house LightCycler real-time PCR (LC-PCR) assays. Specimens initially positive by the BDPT system were retested by our LC-PCR assays. Our results for C. trachomatis testing indicate a 91.2% agreement when the results of 114 clinical specimens, initially positive by BDPT over a wide range of method-other-than-acceleration (MOTA) scores, were retested by our LC-PCR assay. The agreement between the two systems improved to 96% when only MOTA scores of >30,000 were retested by the LC-PCR assay. The overall agreement between the two systems for Neisseria gonorrhoeae detection from 155 clinical specimens was only 77.4%, with agreement particularly low (24.1%) for MOTA scores ranging from 2,000 to 19,999. Repeat testing of specimens with the BDPT only closely correlated with that seen by others demonstrating that reproducibility of the BDPT system for specimens initially within the MOTA score range from 2,000 to 9,999 is problematic, especially for Neisseria gonorrhoeae testing. With our study, we proposed an algorithm for C. trachomatis and N. gonorrhoeae testing which involves screening with the BDPT system followed by selective use of our in-house LC-PCR assays.
Authors:
Michael G Koenig; Sandra L Kosha; Brandon L Doty; David G Heath
Related Documents :
20622219 - Development of a panel of multiplex real-time polymerase chain reaction assays for simu...
19679239 - Real-time polymerase chain reaction detection of cryptococcus neoformans and cryptococc...
19944879 - Winthrop-university hospital infectious disease division's swine influenza (h1n1) pneum...
18949349 - Rickettsial spotted fever in capoeirão village, itabira, minas gerais, brazil.
9256959 - Comparison of nested polymerase chain reaction, virus isolation, and fluorescent antibo...
16519599 - Comparison of the taqman and lightcycler systems in pharmacogenetic testing: evaluation...
24342479 - Evaluation of the accuracy and ease of use of a rapid hiv-1 antibody test performed by ...
16792649 - How low should you go: novel device for nail trephination.
10026439 - Assessment of exocrine pancreatic dysfunction in chronic pancreatitis.
Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  42     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-07     Completed Date:  2005-02-02     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5751-6     Citation Subset:  IM    
Affiliation:
Landstuhl Regional Medical Center, Auf Dem Kirchberg, Attn: Virology, Gebaeude 3738, Zimmer 204, 66849 Landstuhl, Germany. michael.koenig@lnd.amedd.army.mil
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Algorithms
Chlamydia Infections / diagnosis*,  microbiology
Chlamydia trachomatis / genetics,  isolation & purification*
Female
Gonorrhea / diagnosis*,  microbiology
Humans
Male
Military Personnel
Neisseria gonorrhoeae / genetics,  isolation & purification*
Nucleic Acid Amplification Techniques / methods*
Polymerase Chain Reaction / methods*
Reagent Kits, Diagnostic
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Reagent Kits, Diagnostic
Comments/Corrections
Comment In:
J Clin Microbiol. 2005 Apr;43(4):2036-7   [PMID:  15815057 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Diarrhea caused by rotavirus in children less than 5 years of age in Hanoi, Vietnam.
Next Document:  Use of highly variable intergenic spacer sequences for multispacer typing of Rickettsia conorii stra...