| Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl-ethanolamine in plasma. | |
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MedLine Citation:
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PMID: 1882709 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A competitive chemiluminescent immunoassay for quantitation of muramyl tripeptide phosphatidyl-ethanolamine (MTP-PE) in plasma has been developed. The assay is based on the use of an acridinium ester-labelled analogue of muramyl tripeptide and a rabbit antiserum. It includes an overnight incubation and a separation with a second antibody covalently coupled to paramagnetic particles. The sensitivity of detection is 0.012 nmol/l, the assay working range is 0.1-5 nmol/l, and the inter-assay CVs are less than or equal to 10%. Using up to 6000-fold sample dilutions, a wide working range (0.1-30,000 nmol/l) is obtained. Rat plasma samples were collected during and one day after intravenous infusion of MTP-PE. Following infusion, the concentrations in plasma declined multiphasically. Half-life time was 0.37 h +/- 0.03 (mean +/- SD, alpha phase) and 1.76 h +/- 0.08 (mean +/- SD, beta phase), clearance and volume of distribution were 0.09 +/- 0.02 l/h x kg (mean +/- SD) and 0.06 +/- 0.01 l/kg (mean +/- SD) respectively. The use of an acridinium ester as a chemiluminescent (CL) label overcomes the problems associated with reagents of limited shelf-life. |
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Authors:
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B Gay; H Towbin; C Schnell; K Einsle; P Graf; D Gygax |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bioluminescence and chemiluminescence Volume: 6 ISSN: 0884-3996 ISO Abbreviation: J. Biolumin. Chemilumin. Publication Date: 1991 Apr-Jun |
Date Detail:
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Created Date: 1991-09-27 Completed Date: 1991-09-27 Revised Date: 2006-05-17 |
Medline Journal Info:
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Nlm Unique ID: 8612490 Medline TA: J Biolumin Chemilumin Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 73-80 Citation Subset: IM |
Affiliation:
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Pharma Research and Development, CIBA-GEIGY AG, Basle, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetylmuramyl-Alanyl-Isoglutamine
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analogs & derivatives*,
blood,
immunology,
standards Animals Cross Reactions Immunoassay / methods* Luminescent Measurements* Male Phosphatidylethanolamines / blood*, immunology, standards Rats Reference Standards |
| Chemical | |
Reg. No./Substance:
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0/Phosphatidylethanolamines; 53678-77-6/Acetylmuramyl-Alanyl-Isoglutamine; 83461-56-7/muramylNAc-Ala-isoGln-Lys-tripeptide-PE |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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