Document Detail


Direct chemiluminescence immunoassay (CLIA) for muramyl tripeptide phosphatidyl-ethanolamine in plasma.
MedLine Citation:
PMID:  1882709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A competitive chemiluminescent immunoassay for quantitation of muramyl tripeptide phosphatidyl-ethanolamine (MTP-PE) in plasma has been developed. The assay is based on the use of an acridinium ester-labelled analogue of muramyl tripeptide and a rabbit antiserum. It includes an overnight incubation and a separation with a second antibody covalently coupled to paramagnetic particles. The sensitivity of detection is 0.012 nmol/l, the assay working range is 0.1-5 nmol/l, and the inter-assay CVs are less than or equal to 10%. Using up to 6000-fold sample dilutions, a wide working range (0.1-30,000 nmol/l) is obtained. Rat plasma samples were collected during and one day after intravenous infusion of MTP-PE. Following infusion, the concentrations in plasma declined multiphasically. Half-life time was 0.37 h +/- 0.03 (mean +/- SD, alpha phase) and 1.76 h +/- 0.08 (mean +/- SD, beta phase), clearance and volume of distribution were 0.09 +/- 0.02 l/h x kg (mean +/- SD) and 0.06 +/- 0.01 l/kg (mean +/- SD) respectively. The use of an acridinium ester as a chemiluminescent (CL) label overcomes the problems associated with reagents of limited shelf-life.
Authors:
B Gay; H Towbin; C Schnell; K Einsle; P Graf; D Gygax
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bioluminescence and chemiluminescence     Volume:  6     ISSN:  0884-3996     ISO Abbreviation:  J. Biolumin. Chemilumin.     Publication Date:    1991 Apr-Jun
Date Detail:
Created Date:  1991-09-27     Completed Date:  1991-09-27     Revised Date:  2006-05-17    
Medline Journal Info:
Nlm Unique ID:  8612490     Medline TA:  J Biolumin Chemilumin     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  73-80     Citation Subset:  IM    
Affiliation:
Pharma Research and Development, CIBA-GEIGY AG, Basle, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Acetylmuramyl-Alanyl-Isoglutamine / analogs & derivatives*,  blood,  immunology,  standards
Animals
Cross Reactions
Immunoassay / methods*
Luminescent Measurements*
Male
Phosphatidylethanolamines / blood*,  immunology,  standards
Rats
Reference Standards
Chemical
Reg. No./Substance:
0/Phosphatidylethanolamines; 53678-77-6/Acetylmuramyl-Alanyl-Isoglutamine; 83461-56-7/muramylNAc-Ala-isoGln-Lys-tripeptide-PE

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