Document Detail


Direct cell encapsulation in biodegradable and functionalizable carboxybetaine hydrogels.
MedLine Citation:
PMID:  22591611     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Hydrogels provide three-dimensional (3D) frames with tissue-like elasticity and high water content for tissue scaffolds. They were commonly prepared from macromers such as poly(ethylene glycol) diacrylate (PEGDA) via free radical polymerization and used to encapsulate cells. Here, we report the direct encapsulation of cells into hydrogels using a low-toxic and water-soluble monomer, carboxybetaine methacrylate (CBMA), via redox polymerization. A disulfide-containing crosslinker was added to form a biodegradable carboxybetaine (CB) hydrogel, which can be self-degraded as cells grow or degraded in an accelerating way via adding of a cysteine-contained medium NIH-3T3 cells encapsulated in the CB hydrogel formed spherical aggregates that were recovered from hydrogel erosion. Furthermore, an RGD-containing peptide was also added to improve cell adhesion on the two-dimensional (2D) hydrogel surface and promote cell proliferation in the 3D hydrogel. The non-cytotoxic and biodegradable CB hydrogel with additional cell-adhesion moieties provides an excellent 3D environment for cell growth as tissue scaffolds.
Authors:
Hsiu-Wen Chien; Wei-Bor Tsai; Shaoyi Jiang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-14
Journal Detail:
Title:  Biomaterials     Volume:  -     ISSN:  1878-5905     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.
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