Document Detail

Direct survival role of vascular endothelial growth factor (VEGF) on rat ovarian follicular cells.
MedLine Citation:
PMID:  20417686     Owner:  NLM     Status:  MEDLINE    
The aim of the present work was to analyze the direct effect of VEGF in follicular cell proliferation, apoptosis and activation of the PI3K/AKT and ERK/MEK signaling pathways in early antral follicles or granulosa cells. Antral follicles or granulosa cells were isolated from prepubertal female Sprague Dawley rats treated with DES.VEGF directly stimulates follicular cell proliferation and it also decreases apoptosis by inhibiting caspase 3 activation. In addition, VEGF increases the proliferation and inhibits the apoptosis of isolated granulosa cells in culture. VEGF activates the PI3K/AKT pathway evidenced by an increase in AKT phosphorylation levels and induces the phosphorylation of ERK1/2 in cultured antral follicles. These results demonstrate for the first time that VEGF has a proliferative and cytoprotective role in early antral follicles and in granulosa cells isolated from DES treated prepubertal rats and suggest that PI3K/AKT and ERK/MEK signaling pathways are involved in these processes.
Griselda Irusta; Dalhia Abramovich; Fernanda Parborell; Marta Tesone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-24
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  325     ISSN:  1872-8057     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  93-100     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.
Instituto de Biología y Medicina Experimental (IByME) - CONICET, Buenos Aires, Argentina.
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MeSH Terms
Apoptosis / drug effects,  physiology
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Enzyme Activation / drug effects
Extracellular Signal-Regulated MAP Kinases / metabolism
Granulosa Cells / drug effects,  metabolism,  physiology
Oncogene Protein v-akt / metabolism
Ovarian Follicle / drug effects,  metabolism,  physiology*
Rats, Sprague-Dawley
Sexual Maturation / physiology
Signal Transduction / drug effects
Vascular Endothelial Growth Factor A / pharmacology,  physiology*
Reg. No./Substance:
0/Vascular Endothelial Growth Factor A; 0/vascular endothelial growth factor A, rat; EC Protein v-akt; EC Signal-Regulated MAP Kinases

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