| Direct observation to chemokine receptor 5 on T-lymphocyte cell surface using fluorescent metal nanoprobes. | |
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MedLine Citation:
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PMID: 20705055 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemokine receptor 5 (CCR5) is a cell surface protein required for HIV-1 infection. It is important to detect the amount and observe the spatial distribution of the CCR5 receptors on the cell surfaces. In this report, we describes the metal nanoparticles which were specially designed as molecular fluorescent probes for imaging of CCR5 receptors on the T-lymphocytic PM1 cell surfaces. These CCR5 monoclonal antibodies (mAbs) metal complexes were prepared by labeling mAbs with Alexa Fluor 680 followed by covalent binding the labeled mAbs on the 20 nm silver nanoparticles. Compared with the labeled mAbs without metal, the mAb-metal complexes were found to display enhanced emission intensity and shortened lifetime due to interactions between fluorophores and metal. The mAb-metal complexes were incubated with the PM1 cell lines. The confocal fluorescent intensity and lifetime cell images were recorded on single cells. It was observed that the mAb-metal complexes could be clearly distinguished from the cellular autofluorescence. By analyzing a pool of cell images, we observed that most CCR5 receptors appeared as clusters on the cell surfaces. The fluorophore-metal complexes developed in this report are generally useful for detection of cell surface receptors and provide a new class of probe to study the interaction between the CCR5 receptors with viral gp120 during HIV infections. |
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Authors:
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Jian Zhang; Yi Fu; Ge Li; Kazimierz Nowaczyk; Richard Y Zhao; Joseph R Lakowicz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-08-10 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 400 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-13 Completed Date: 2010-10-11 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 111-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, Baltimore, MD 21201, USA. jian@cfs.bioment.umaryland.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal
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immunology Cell Line Cell Membrane / chemistry, immunology* HIV Envelope Protein gp120 / immunology Humans Metal Nanoparticles / chemistry* Molecular Probes Receptors, CCR5 / analysis*, immunology Spectrometry, Fluorescence / methods* T-Lymphocytes / chemistry, immunology*, virology |
| Grant Support | |
ID/Acronym/Agency:
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CA134386/CA/NCI NIH HHS; EB006521/EB/NIBIB NIH HHS; EB009509/EB/NIBIB NIH HHS; HG-002655/HG/NHGRI NIH HHS; HG005090/HG/NHGRI NIH HHS; R01 EB006521-03/EB/NIBIB NIH HHS; R01 HG002655-08/HG/NHGRI NIH HHS; R21 CA134386-02/CA/NCI NIH HHS; R21 EB009509-01A2/EB/NIBIB NIH HHS; R21 EB009509-02/EB/NIBIB NIH HHS; R21 HG005090-03/HG/NHGRI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/HIV Envelope Protein gp120; 0/Molecular Probes; 0/Receptors, CCR5 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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