| Direct Detection and Genotyping of KPC Carbapenemases from Urine using a new DNA Microarray Test. | |
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MedLine Citation:
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PMID: 23035190 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Klebsiella pneumoniae carbapenemases (KPC) are considered a serious threat to antibiotic therapy as they confer resistance to carbapenems, which are used to treat Extended-spectrum beta-lactamase (ESBL) producing bacteria. Here, we describe the development and evaluation of a DNA microarray for detection and genotyping of KPC genes (bla(KPC)) within 5 hours. To test the whole assay procedure (DNA extraction + DNA microarray assay) directly from clinical specimen, we compared two commercial DNA extraction kits (QIAprep Spin Miniprep Kit (Qiagen), Urine Bacterial DNA Isolation Kit (Norgen)) for the direct DNA extraction from urine samples (dilution series spiked in human urine). A reliable SNP typing from 1×10(5) CFU/mL urine was demonstrated for Escherichia coli (Qiagen and Norgen) and 80 CFU/mL urine on average for K. pneumoniae (Norgen). The study presents for the first time the combination of a new KPC-microarray with commercial sample preparation for the detection and genotyping of microbial pathogens directly from clinical specimen which paves the way towards tests providing epidemiological and diagnostic data enabling better antimicrobial stewardship. |
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Authors:
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Harald Peter; Kathrine Berggrav; Peter Thomas; Yvonne Pfeifer; Wolfgang Witte; Kate Templeton; Till T Bachmann |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: - ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Division of Pathway Medicine, University of Edinburgh, Medical School, Chancellor's Building, Little France Crescent, Edinburgh EH16 4SB, United Kingdom. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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