| Direct derivatization and rapid GC-MS screening of nerve agent markers in aqueous samples. | |
| | |
MedLine Citation:
|
PMID: 20701266 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
A rapid screening and identification method based on derivatization and gas chromatography mass spectrometry (GC-MS) has been developed for the detection of alkylphosphonic acids (APAs), the degradation products of organophosphorus nerve agents. The novel method described involves rapid (5 min) and direct derivatization of 25 microL aqueous sample using highly fluorinated phenyldiazomethane reagents (e.g., 1-(diazomethyl)-3,5-bis(trifluoromethyl)benzene). The APA derivatives are then screened by GC-MS negative ion chemical ionization (NICI) and identified by electron ionization (EI) mode. The conditions for the derivatization were optimized using statistical experimental design and multivariate data analysis. Method robustness was evaluated using aqueous samples from an official OPCW Proficiency Test and all APAs present in the sample were conclusively identified. Limits of detection for rapid screening using SIM NICI were between 5 and 10 ng/mL APA in aqueous sample, and for identification using full scan EI 100 ng/mL. |
| | |
Authors:
|
Raja Subramaniam; Crister Astot; Lars Juhlin; Calle Nilsson; Anders Ostin |
Related Documents
:
|
15808016 - Analytical monitoring of trinitrotoluene metabolites in urine by gc-ms. part i. semiqua... 16131076 - High-throughput data analysis for detecting and identifying differences between samples... 15865176 - Analysis of chlorinated, sulfochlorinated and sulfonamide derivatives of n-tetradecane ... 4054586 - Identification of estradiol in the ovaries of the silkworm, bombyx mori. 2693086 - Crossed affino-immunoelectrophoresis or affino-blotting with lectins: advantages and li... 12367706 - Hplc assay for albendazole and metabolites in human plasma for clinical pharmacokinetic... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Analytical chemistry Volume: 82 ISSN: 1520-6882 ISO Abbreviation: Anal. Chem. Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-31 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 7452-9 Citation Subset: - |
Affiliation:
|
The Swedish Defence Research Agency, FOI CBRN Defence and Security, SE-901 82 Umeå, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Experimental evidence for the nanocage effect in catalysis with hollow nanoparticles.
Next Document: Magnetic nanochains of FeNi3 prepared by a template-free microwave-hydrothermal method.