Document Detail

Direct cellular peptidomics of hypothalamic neurons.
MedLine Citation:
PMID:  21334363     Owner:  NLM     Status:  MEDLINE    
The chemical complexity of cell-to-cell communication has emerged as a fundamental challenge to understanding brain systems. This is certainly true for the hypothalamus, where neuropeptide signals are heterogeneous, localized and dynamic. Thus far, most hypothalamic peptidomic studies have centered on the entire structure; however, recent advances in collection strategies and analytical technologies have enabled direct, high-resolution peptidomic profiles focused on two regions of interest, the suprachiasmatic and supraoptic nuclei, including their sub-regions and individual cells. Suites of peptides now can be identified and probed for function. High spatial and analytical sensitivities reveal that discrete hypothalamic nuclei have distinct peptidomic signatures. Peptidomic discovery not only reveals unanticipated complexity, but also peptides previously unknown that act as key circuit components. Analysis of tissue releasates identifies peptides secreted into the extracellular environment and available for transmitting intercellular signals. Direct sampling techniques define peptide-releasate profiles in spatial, temporal and event-dependent patterns. These approaches are providing remarkable new insights into the complexity of neuropeptidergic cell-to-cell signaling central to neuroendocrine physiology.
Jennifer W Mitchell; Norman Atkins; Jonathan V Sweedler; Martha U Gillette
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2011-02-18
Journal Detail:
Title:  Frontiers in neuroendocrinology     Volume:  32     ISSN:  1095-6808     ISO Abbreviation:  Front Neuroendocrinol     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-09-29     Completed Date:  2012-01-23     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  7513292     Medline TA:  Front Neuroendocrinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  377-86     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Inc. All rights reserved.
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MeSH Terms
Genetic Association Studies
Hypothalamus / chemistry,  metabolism*
Models, Biological
Neurons / chemistry,  metabolism*
Neuropeptides / analysis,  genetics,  metabolism*
Organ Specificity
Proteome / analysis
Proteomics / methods*
Grant Support
HL086870/HL/NHLBI NIH HHS; HL092571/HL/NHLBI NIH HHS; P30 DA018310/DA/NIDA NIH HHS; P30 DA018310/DA/NIDA NIH HHS; P30 DA018310-01/DA/NIDA NIH HHS; P30 DA018310-02/DA/NIDA NIH HHS; P30 DA018310-03/DA/NIDA NIH HHS; P30 DA018310-04/DA/NIDA NIH HHS; P30 DA018310-05/DA/NIDA NIH HHS; P30 DA018310-06/DA/NIDA NIH HHS; P30 DA018310-07/DA/NIDA NIH HHS; P30 DA018310-08/DA/NIDA NIH HHS; R01 HL092571/HL/NHLBI NIH HHS; R01 HL092571-01A1/HL/NHLBI NIH HHS; R01 HL092571-02/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Neuropeptides; 0/Proteome

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