| Dipyridamole suppresses high glucose-induced osteopontin secretion and mRNA expression in rat aortic smooth muscle cells. | |
| | |
MedLine Citation:
|
PMID: 20453393 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Diabetic patients are frequently afflicted with medial artery calcification, a predictor of cardiovascular mortality. Diabetes induced the expression of osteopontin in arterial vasculature, which is an indicator of disease progression in artery calcification and vascular stiffness. Signal transduction and strategies that suppress high glucose-induced osteopontin expression in arterial vascular smooth muscle cells is investigated. METHODS AND RESULTS: The incubation of rat aortic smooth muscle cells under high glucose concentration increased osteopontin protein secretion and mRNA expression. Treatment with dipyridamole decreased high glucose-induced osteopontin expression and secretion. Dipyridamole decreased glucose-induced osteopontin through inhibition of phosphodiesterase, thereby increasing intracellular levels of adenosine-3',5'-cyclic monophosphate (cAMP) and guanosine-3',5'-cyclic monophosphate (cGMP), and increased thioredoxin expression to inhibit the reactive oxygen species (ROS) system. Induction of osteopontin was reversed when cells were pretreated with N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H89, cAMP-dependent protein kinase inhibitor), KT5823 (cGMP-dependent protein kinase inhibitor), or dinitrochlorobenzene (thioredoxin reductase inhibitor). The antioxidant, N-acetyl-L-cysteine, suppressed glucose-induced osteopontin expression by decreasing ROS concentration. Both H89 and KT5823 downregulated thioredoxin expression. CONCLUSIONS: These results suggest a novel effect for dipyridamole to suppress high glucose-induced osteopontin protein secretion and mRNA expression. Dipyridamole has antioxidant properties and a phosphodiesterase inhibitor activity, which might be useful to ameliorate diabetic vasculopathy and its cardiovascular complications. |
| | |
Authors:
|
Ming-Song Hsieh; Wen-Bin Zhong; Shu-Chuan Yu; John Yi-Chung Lin; Wei-Ming Chi; Horng-Mo Lee |
Related Documents
:
|
17056753 - Glucose uptake pathway-specific regulation of synthesis of neotrehalosadiamine, a novel... 9806883 - Regulation of mammalian pyruvate dehydrogenase alpha subunit gene expression by glucose... 15682443 - Malt lymphoma with t(14;18)(q32;q21)/igh-malt1 is characterized by strong cytoplasmic m... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-20 |
Journal Detail:
|
Title: Circulation journal : official journal of the Japanese Circulation Society Volume: 74 ISSN: 1347-4820 ISO Abbreviation: Circ. J. Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-27 Completed Date: 2010-09-07 Revised Date: 2011-02-18 |
Medline Journal Info:
|
Nlm Unique ID: 101137683 Medline TA: Circ J Country: Japan |
Other Details:
|
Languages: eng Pagination: 1242-50 Citation Subset: IM |
Affiliation:
|
Department of Laboratory Medicine, Shuang-Ho Hospital, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antioxidants Aorta / cytology* Biological Markers / analysis Calcinosis Cells, Cultured Diabetic Angiopathies Dipyridamole / pharmacology* Glucose / pharmacology* Humans Muscle, Smooth, Vascular / secretion* Myocytes, Smooth Muscle / cytology, secretion Osteopontin / analysis, metabolism, secretion* Phosphodiesterase Inhibitors RNA, Messenger / analysis* Rats Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antioxidants; 0/Biological Markers; 0/Phosphodiesterase Inhibitors; 0/RNA, Messenger; 0/Reactive Oxygen Species; 106441-73-0/Osteopontin; 50-99-7/Glucose; 58-32-2/Dipyridamole |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Assessment of mechanical properties of common carotid artery in Takayasu's arteritis using velocity ...
Next Document: Intriguing peri-strut low-intensity area detected by optical coherence tomography after coronary ste...