| Diphosphonucleotide phosphatase/phosphodiesterase (PPD1) from yellow lupin (Lupinus luteus L.) contains an iron-manganese center. | |
| | |
MedLine Citation:
|
PMID: 19755125 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Yellow lupin diphosphonucleotide phosphatase/phosphodiesterase (PPD1) represents a novel group of enzymes. Here we report that it possesses one iron atom and one manganese atom (1:1 molar ratio) per subunit. The enzyme exhibits visible absorption maximum at approximately 530 nm. Prolonged oxidation of PPD1 leads to loss of the charge-transfer band and catalytic activity, whereas after reduction PPD1 remains active. Replacement of conserved amino-acid residues coordinating metals results in the loss of enzymatic activity. Despite low amino-acid sequence homology of PPD1 to well-characterized approximately 55-kDa purple acid phosphatases, their overall fold, topology of active center and metal content are highly similar. |
| | |
Authors:
|
Mariusz Olczak; Justyna Ciuraszkiewicz; Halina Wójtowicz; Dorota Maszczak; Teresa Olczak |
Related Documents
:
|
9027995 - Development of a protein phosphatase-based assay for the detection of phosphatase inhib... 1252995 - Phosphopantothenic acid--the major product of pantothenic acid accumulation and a signi... 2797865 - Megasomes as the targets of leucine methyl ester in leishmania amazonensis amastigotes. 9797305 - Comparison of the thermostability properties of three acid phosphatases from molds: asp... 6735685 - Further studies on the physico-chemical properties of rhizopus nodosus acid lipase. 201655 - Oscillations and cell development in dictyostelium discoideum stimulated by folic acid ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-13 |
Journal Detail:
|
Title: FEBS letters Volume: 583 ISSN: 1873-3468 ISO Abbreviation: FEBS Lett. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-10-05 Completed Date: 2009-11-13 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 3280-4 Citation Subset: IM |
Affiliation:
|
Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland. Mariusz.Olczak@biotech.uni.wroc.pl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Conserved Sequence Iron / chemistry, metabolism* Lupinus / enzymology* Manganese / chemistry, metabolism* Mass Spectrometry Models, Molecular Phosphoric Diester Hydrolases / chemistry, metabolism* Protein Conformation Pyrophosphatases / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
|
7439-89-6/Iron; 7439-96-5/Manganese; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.6.1.-/Pyrophosphatases; EC 3.6.1.-/diphosphonucleotide phosphatase-phosphodiesterase 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Melanocortin 1 receptor mutations impact differentially on signalling to the cAMP and the ERK mitoge...
Next Document: The scaling of dose with host body mass and the determinants of success in experimental cercarial in...