Document Detail


Diphosphonucleotide phosphatase/phosphodiesterase (PPD1) from yellow lupin (Lupinus luteus L.) contains an iron-manganese center.
MedLine Citation:
PMID:  19755125     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Yellow lupin diphosphonucleotide phosphatase/phosphodiesterase (PPD1) represents a novel group of enzymes. Here we report that it possesses one iron atom and one manganese atom (1:1 molar ratio) per subunit. The enzyme exhibits visible absorption maximum at approximately 530 nm. Prolonged oxidation of PPD1 leads to loss of the charge-transfer band and catalytic activity, whereas after reduction PPD1 remains active. Replacement of conserved amino-acid residues coordinating metals results in the loss of enzymatic activity. Despite low amino-acid sequence homology of PPD1 to well-characterized approximately 55-kDa purple acid phosphatases, their overall fold, topology of active center and metal content are highly similar.
Authors:
Mariusz Olczak; Justyna Ciuraszkiewicz; Halina Wójtowicz; Dorota Maszczak; Teresa Olczak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-13
Journal Detail:
Title:  FEBS letters     Volume:  583     ISSN:  1873-3468     ISO Abbreviation:  FEBS Lett.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-05     Completed Date:  2009-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  3280-4     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw, Tamka 2, 50-137 Wroclaw, Poland. Mariusz.Olczak@biotech.uni.wroc.pl
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Conserved Sequence
Iron / chemistry,  metabolism*
Lupinus / enzymology*
Manganese / chemistry,  metabolism*
Mass Spectrometry
Models, Molecular
Phosphoric Diester Hydrolases / chemistry,  metabolism*
Protein Conformation
Pyrophosphatases / chemistry,  metabolism*
Chemical
Reg. No./Substance:
7439-89-6/Iron; 7439-96-5/Manganese; EC 3.1.4.-/Phosphoric Diester Hydrolases; EC 3.6.1.-/Pyrophosphatases; EC 3.6.1.-/diphosphonucleotide phosphatase-phosphodiesterase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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