Document Detail


Dipeptidyl Peptidase (DPP) IV Inhibitor Attenuates Kidney Injury in Streptozotocin Induced Diabetic Rats.
MedLine Citation:
PMID:  22025647     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Dipeptidyl peptidase (DPP) IV inhibitors are likely beneficial for treating diabetic complication and modulating glucagon-like peptide-1 receptor (GLP-1R) expression. The aim of this study was to determine if the DPP IV inhibitor, LAF237, has renoprotective qualities in streptozotocin-induced diabetic rats. Diabetic and non-diabetic rats were treated with an oral dose of 4 or 8mg/kg/day LAF237 or placebo for 24 weeks, and renal injury was observed by light and electron microscopy. We also assessed DPP IV activity, GLP-1 level, cAMP and 8-OhdG excretion, and GLP-1R, caspase 3 and transforming growth factor (TGF)-β1 expression. LAF237 significantly decreased proteinuria, albuminuria and urinary albumin/creatinine ratio, improved creatinine clearance, and dose-dependently inhibited interstitial expansion, glomerulosclerosis and the thickening of the glomerular basement membrane in diabetic rats. Notably, LAF237 markedly down-regulated DPP IV activity and increased GLP-1 levels, which likely prevented oxidative DNA damage and renal cell apoptosis by activating the GLP-1R and modulating cAMP. Renoprotection was also associated with a reduction in TGF-β1 overexpression. Our study suggests that DPP IV inhibitors may ameliorate diabetic nephropathy as well as reduce the overproduction of TGF-β1. The observed renoprotection is probably attributable to inhibition of DPP IV activity, mimicking incretin action and activating the GLP-1R.
Authors:
Wei Jing Liu; Shu Hua Xie; Yu Nin Liu; Won Kim; Heung Yong Jin; Sung Kwang Park; Yi Ming Shao; Tae Sun Park
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-24
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  -     ISSN:  1521-0103     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 Nephrology;
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