| Dipeptide synthesis by an aminopeptidase from Streptomyces septatus TH-2 and its application to synthesis of biologically active peptides. | |
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MedLine Citation:
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PMID: 16751535 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dipeptide synthesis by aminopeptidase from Streptomyces septatus TH-2 (SSAP) was demonstrated using free amino acid as an acyl donor and aminoacyl methyl ester as an acyl acceptor in 98% methanol (MeOH). SSAP retained its activity after more than 100 h in 98% MeOH, and in the case of phenylalanyl-phenylalanine methyl ester synthesis, the enzyme reaction reached equilibrium when more than 50% of the free phenylalanine was converted to the product. In an investigation of the specificity of SSAP toward acyl donors and acyl acceptors, SSAP showed a broad specificity toward various free amino acids and aminoacyl methyl esters. Furthermore, we applied SSAP to the synthesis of several biologically active peptides, such as aspartyl-phenylalanine, alanyl-tyrosine, and valyl-tyrosine methyl esters. |
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Authors:
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Jiro Arima; Yoshiko Uesugi; Misugi Uraji; Masaki Iwabuchi; Tadashi Hatanaka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 72 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-05 Completed Date: 2006-08-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 4225-31 Citation Subset: IM |
Affiliation:
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Research Institute for Biological Sciences (RIBS), Okayama, 7549-1 Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminopeptidases
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metabolism* Chromatography, High Pressure Liquid Dipeptides / biosynthesis* Kinetics Methanol / metabolism Streptomyces / enzymology* Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Dipeptides; 67-56-1/Methanol; EC 3.4.11.-/Aminopeptidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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